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Literature summary for 3.2.1.55 extracted from

  • Kurakake, M.; Kanbara, Y.; Murakami, Y.
    Characteristics of alpha-L-arabinofuranosidase from Streptomyces sp I10-1 for production of L-arabinose from corn hull arabinoxylan (2014), Appl. Biochem. Biotechnol., 172, 2650-2660.
    View publication on PubMed
Search for more literature for 3.2.1.55

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparisons Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 x * 45248, sequence calculation, x * 31000, SDS-PAGE Streptomyces sp.
45248
-
 x * 45248, sequence calculation, x * 31000, SDS-PAGE Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp. B6ZGN5 isolated from soil
-
Streptomyces sp. I10-1 B6ZGN5 isolated from soil
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces sp. 4-nitrophenol + L-arabinofuranose
-
 ?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces sp. I10-1 4-nitrophenol + L-arabinofuranose
-
 ?
corn hull arabinoxylan + H2O efficiently produces L-arabinose from high arabinose-content corn hull arabinoxylan, that has a ratio of arabinose to xylose of 0.6 Streptomyces sp. ?
-
 ?
corn hull arabinoxylan + H2O efficiently produces L-arabinose from high arabinose-content corn hull arabinoxylan, that has a ratio of arabinose to xylose of 0.6 Streptomyces sp. I10-1 ?
-
 ?
additional information substrate specificity, overview. No activity with 4-nitrophenyl-alpha-L-arabinopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, and 4-nitrophenyl-beta-D-xylopyranoside, and arabinogalactan from larch Streptomyces sp. ?
-
 ?
additional information substrate specificity, overview. No activity with 4-nitrophenyl-alpha-L-arabinopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, and 4-nitrophenyl-beta-D-xylopyranoside, and arabinogalactan from larch Streptomyces sp. I10-1 ?
-
 ?
sugar beet arabinan + H2O
-
 Streptomyces sp. ?
-
 ?
sugar beet arabinan + H2O
-
 Streptomyces sp. I10-1 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 45248, sequence calculation, x * 31000, SDS-PAGE Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 at pH 5.0 Streptomyces sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60 activity range, profile overview Streptomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 purified recombinant enzyme, 60 min, stable up to Streptomyces sp.
40
-
 purified recombinant enzyme, 60 min, 90% activity remaining Streptomyces sp.
50
-
 purified recombinant enzyme, 60 min, 40% activity remaining Streptomyces sp.
60
-
 purified recombinant enzyme, 60 min, no activity remaining Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 at 40°C Streptomyces sp.

pH Range

pH Minimum pH Maximum Comment Organism
3 10 activity range, profile overview Streptomyces sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
3
-
 purified recombinant enzyme, 60 min, no activity remaining Streptomyces sp.
4
-
 purified recombinant enzyme, 60 min, 50% activity remaining Streptomyces sp.
5 7 purified recombinant enzyme, 60 min, full activity remaining Streptomyces sp.
8 9 purified recombinant enzyme, 60 min, 80% activity remaining Streptomyces sp.
10 11 purified recombinant enzyme, 60 min, 60% activity remaining Streptomyces sp.

General Information

General Information Comment Organism
evolution the enzyme belongs to the alpha-L-AF C superfamily, which is associated with glycoside hydrolase family 51, but the enzyme's properties are unique Streptomyces sp.
additional information tryptic peptide mass fingerprinting, overview Streptomyces sp.