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Literature summary for 3.2.1.55 extracted from

  • Alias, N.; Mahadi, N.; Murad, A.; Bakar, F.; Rabu, A.; Illias, R.
    Expression optimisation of recombinant alpha-L-arabinofuranosidase from Aspergillus niger ATCC 120120 in Pichia pastoris and its biochemical characterization (2011), Afr. J. Biotechnol., 10, 6700-6710.
No PubMed abstract available
Search for more literature for 3.2.1.55

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 149.58% activity at 1 mM Aspergillus niger

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris X-33 cells Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 34.37% residual activity at 1 mM Aspergillus niger
DMSO 87.55% residual activity at 5% (v/v) Aspergillus niger
Fe2+ 34.37% residual activity at 1 mM Aspergillus niger
additional information the enzyme activity is relatively unaffected by EDTA Aspergillus niger
Urea 84.86% residual activity at 1 M Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.93
-
 4-nitrophenyl alpha-L-arabinofuranoside in 50 mM acetate buffer, pH 5.0, at 50°C Aspergillus niger

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 113.85% activity at 1% (v/v) Aspergillus niger
Mn2+ 136.48% activity at 1 mM Aspergillus niger
additional information the enzyme activity is relatively unaffected by Ca2+, CO2+, Mg2+ and EDTA Aspergillus niger
Na+ 111.17% activity at 1 mM Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65334
-
 x * 65334, calculated from amino acid sequence Aspergillus niger
66000
-
 x * 66000, the purified recombinant abfA before and after treatment with PNGase F migrated by SDS-PAGE has relative molecular masses of about 83000 and 66000 Da, respectively Aspergillus niger
83000
-
 x * 83000, the purified recombinant abfA before and after treatment with PNGase F migrated by SDS-PAGE has relative molecular masses of about 83000 and 66000 Da, respectively Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger D6QST5
-
-
Aspergillus niger ATCC 120120 D6QST5
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the enzyme is N-linked glycosylated Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Aspergillus niger 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Aspergillus niger ATCC 120120 4-nitrophenol + alpha-L-arabinofuranose
-
 ?

Subunits

Subunits Comment Organism
? x * 65334, calculated from amino acid sequence Aspergillus niger
? x * 66000, the purified recombinant abfA before and after treatment with PNGase F migrated by SDS-PAGE has relative molecular masses of about 83000 and 66000 Da, respectively Aspergillus niger
? x * 83000, the purified recombinant abfA before and after treatment with PNGase F migrated by SDS-PAGE has relative molecular masses of about 83000 and 66000 Da, respectively Aspergillus niger

Synonyms

Synonyms Comment Organism
AbfA
-
 Aspergillus niger
alpha-L-arabinofuranosidase
-
 Aspergillus niger
alpha-L-arabinofuranoside arabinofuranohydrolase
-
 Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 in 50 mM acetate buffer (pH 5.0) Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 50 the enzyme exhibits stability at temperatures up to 50°C, retaining 90% of its activity after pre-incubation at 40°C for 30 min. Higher temperatures result in a complete decrease in the enzyme activity Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 6 the enzyme is stable from pH 3.0 to 6.0 with a gradual loss of activity at higher and lower pH values. The enzyme is slowly inactivated at pH levels above 7.0 Aspergillus niger

Expression

Organism Comment Expression
Aspergillus niger gene abfA is expressed as an active enzyme at 28°C when cultured in BMMY medium (pH 6.0) and induced with 2% (v/v) methanol every 24 h. Maximum activity is observed 5 days after induction up