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Literature summary for 3.2.1.55 extracted from

  • Sorensen, H.R.; Pedersen, S.; Jorgensen, C.T.; Meyer, A.S.
    Enzymatic hydrolysis of wheat arabinoxylan by a recombinant "minimal" enzyme cocktail containing beta-xylosidase and novel endo-1,4-beta-xylanase and alpha-L-arabinofuranosidase activities (2007), Biotechnol. Prog., 23, 100-107.
    View publication on PubMed
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Application

Application Comment Organism
degradation enzymatic hydrolysis of wheat arabinoxylan. Treatment of water-soluble and water-insoluble wheat arabinoxylan with an enzyme cocktail containing a 20%:20%:20%:40% mixture and a 25%:25%:25%:25% mixture, respectively, of the GH43 alpha-L-arabinofuranosidase from Humicola insolens (Abf II), the GH51 alpha-L-arabinofuranosidase from Meripilus giganteus (Abf III), a GH10 endo-1,4-beta-xylanase from Humicola insolens (Xyl III), and a GH3 beta-xylosidase from Trichoderma reesei releases 322 mg of arabinose and 512 mg of xylose per gram of water-soluble wheat arabinoxylan dry matter and 150 mg of arabinose and 266 mg of xylose per gram of water-insoluble wheat arabinoxylan dry matter after 24 h at pH 5, 50°C. A 10%:40%:50% mixture of Abf II, Abf III, and beta-xyl releases 56 mg of arabinose and 91 mg of xylose per gram of vinasse dry matter after 24 h at pH 5, 50°C. The optimal dosages of the enzyme cocktails are determined to be 0.4, 0.3, and 0.2 g enzyme protein per kilogram of substrate dry matter for the water-soluble wheat arabinoxylan, the water-insoluble wheat arabinoxylan, and the vinasse, respectively Humicola insolens
degradation enzymatic hydrolysis of wheat arabinoxylan. Treatment of water-soluble and water-insoluble wheat arabinoxylan with an enzyme cocktail containing a 20%:20%:20%:40% mixture and a 25%:25%:25%:25% mixture, respectively, of the GH43 alpha-L-arabinofuranosidase from Humicola insolens (Abf II), the GH51 alpha-L-arabinofuranosidase from Meripilus giganteus (Abf III), a GH10 endo-1,4-beta-xylanase from Humicola insolens (Xyl III), and a GH3 beta-xylosidase from Trichoderma reesei releases 322 mg of arabinose and 512 mg of xylose per gram of water-soluble wheat arabinoxylan dry matter and 150 mg of arabinose and 266 mg of xylose per gram of water-insoluble wheat arabinoxylan dry matter after 24 h at pH 5, 50°C. A 10%:40%:50% mixture of Abf II, Abf III, and beta-xyl releases 56 mg of arabinose and 91 mg of xylose per gram of vinasse dry matter after 24 h at pH 5, 50°C. The optimal dosages of the enzyme cocktails are determined to be 0.4, 0.3, and 0.2 g enzyme protein per kilogram of substrate dry matter for the water-soluble wheat arabinoxylan, the water-insoluble wheat arabinoxylan, and the vinasse, respectively Meripilus giganteus

Organism

Organism UniProt Comment Textmining
Humicola insolens
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-
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Meripilus giganteus
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Synonyms

Synonyms Comment Organism
Abf II
-
 Humicola insolens
Abf III
-
 Meripilus giganteus
alpha-L-arabinofuranosidase II
-
 Humicola insolens
alpha-L-arabinofuranosidase III
-
 Meripilus giganteus