Cloned (Comment) | Organism |
---|---|
expression in Pichia pastori as an active enzyme accumulated in the culture broth, wild-type and mutant enzymes lacking one or both asparagine residues in the N-glycosylation sites N83Q, N202Q and N83Q/N202Q | Aspergillus luchuensis |
Protein Variants | Comment | Organism |
---|---|---|
N202Q | T50-value is 3°C lower than wild-type value. Vmax towards p-nitrophenyl-alpha-L-arabinofuranoside is 0.76fold of wild-type value. No significant effect on kcat/Km with p-nitrophenyl-alpha-L-arabinofuranoside as substrate. Significant decrease in specific activity towards arabinan and debranched arabinan compared to wild-type enzyme | Aspergillus luchuensis |
N83Q | T50-value is 1°C lower than wild-type value. No significant effect on kcat/Km | Aspergillus luchuensis |
N83Q/N202Q | T50-value is 3°C lower than wild-type valueVmax towards p-nitrophenyl-alpha-L-arabinofuranoside is 0.71fold of wild-type value. No significant effect on kcat/K with p-nitrophenyl-alpha-L-arabinofuranoside as substrate. Significant decrease in specific activity towards arabinan and debranched arabinan compared to wild-type enzyme | Aspergillus luchuensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.69 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | pH 4.0, 37°C, mutant enzyme N83Q/N202Q | Aspergillus luchuensis | |
0.86 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | pH 4.0, 37°C, mutant enzyme N202Q | Aspergillus luchuensis | |
1.04 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | pH 4.0, 37°C, wild-type enzyme | Aspergillus luchuensis | |
1.07 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | pH 4.0, 37°C, mutant enzyme N83Q | Aspergillus luchuensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
x * 59000, mutant N83Q/N202Q, SDS-PAGE | Aspergillus luchuensis |
60700 | - |
x * 60700, mutant N83Q, SDS-PAGE | Aspergillus luchuensis |
62000 | - |
x * 62000, mutant N202Q, SDS-PAGE | Aspergillus luchuensis |
64500 | - |
x * 64500, wild-type enzyme, SDS-PAGE | Aspergillus luchuensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus luchuensis | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the native enzyme contains N-linked oligosaccharides. The Asn202-linked oligosaccharide may contribute to thermostability | Aspergillus luchuensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arabinan + H2O | - |
Aspergillus luchuensis | ? | - |
? | |
debranched arabinan + H2O | - |
Aspergillus luchuensis | ? | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Aspergillus luchuensis | p-nitrophenol + L-arabinofuranose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 59000, mutant N83Q/N202Q, SDS-PAGE | Aspergillus luchuensis |
? | x * 60700, mutant N83Q, SDS-PAGE | Aspergillus luchuensis |
? | x * 62000, mutant N202Q, SDS-PAGE | Aspergillus luchuensis |
? | x * 64500, wild-type enzyme, SDS-PAGE | Aspergillus luchuensis |
Synonyms | Comment | Organism |
---|---|---|
AkAbf54 | - |
Aspergillus luchuensis |
alpha-L-arabinofuranosidase 54 | - |
Aspergillus luchuensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
T50-value of mutant enzyme N202Q is 60.1°C | Aspergillus luchuensis |
60 | - |
T50-value of mutant enzyme N83Q/N202Q is 60.1°C | Aspergillus luchuensis |
62 | - |
T50-value of mutant enzyme N83Q is 62.2°C | Aspergillus luchuensis |
63 | - |
T50-value of wild-type enzyme is 63.3°C | Aspergillus luchuensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0694 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | pH 4.0, 37°C, mutant enzyme N83Q/N202Q | Aspergillus luchuensis | |
0.0755 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | pH 4.0, 37°C, mutant enzyme N202Q | Aspergillus luchuensis | |
0.0898 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | pH 4.0, 37°C, mutant enzyme N83Q | Aspergillus luchuensis | |
0.099 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | pH 4.0, 37°C, wild-type enzyme | Aspergillus luchuensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
hydrolytic activity of wild-type enzyme | Aspergillus luchuensis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 7 | stable | Aspergillus luchuensis |