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Literature summary for 3.2.1.54 extracted from
- Characterization of a novel cyclomaltodextrinase expressed from environmental DNA isolated from Bor Khleung hot spring in Thailand (2006), FEMS Microbiol. Lett., 260, 91-99.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli and Pichia pastoris | unidentified |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.4 | - |
alpha-cyclodextrin | - |
unidentified |  |
| 6.1 | - |
beta-cyclodextrin | - |
unidentified | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required for full activity | unidentified | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68000 | - |
SDS-PAGE, size exclusion chromatography | unidentified |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| unidentified | - |
Env Cda13A can be classified into the neopullulanase subfamily consisting of neopullulanases, cyclomaltodextrinases and maltogenic amylases | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| of the recombinant protein by filtration of the supernatant | unidentified |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.1 | - |
starch as substrate | unidentified |
| 0.3 | - |
pullulan as substrate | unidentified |
| 3.4 | - |
gamma-cyclodextrin as substrate | unidentified |
| 14.8 | - |
beta-cyclodextrin as substrate | unidentified |
| 30.7 | - |
alpha-cyclodextrin as substrate | unidentified |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-cyclodextrin + H2O | - |
unidentified | maltose + D-glucose | - |
? | |
| beta-cyclodextrin + H2O | - |
unidentified | maltose + D-glucose | in an approximate molar ratio of 2 to 1 | ? | |
| cyclomaltodextrin + H2O | - |
unidentified | linear maltodextrin | - |
? | |
| gamma-cyclodextrin + H2O | - |
unidentified | maltose + D-glucose | in an approximate molar ratio of 2 to 1 | ? | |
| maltooligosaccharides + H2O | - |
unidentified | D-glucose + ? | - |
? | |
| pullulan + H2O | - |
unidentified | maltotriose + ? | - |
? | |
| starch + H2O | - |
unidentified | D-glucose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
unidentified |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CDase | - |
unidentified |
| cyclomaltodextrinase | - |
unidentified |
| Env cda13A | - |
unidentified |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 55 | - |
unidentified |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | - |
unidentified |
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Release 2023.1 (January 2023)
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