Literature summary for 3.2.1.54 extracted from

Auh, J.H.; Chae, H.Y.; Kim, Y.R.; Shim, K.H.; Yoo, S.H.; Park, K.H.
Modification of rice starch by selective degradation of amylose using alkalophilic Bacillus cyclomaltodextrinase (2006), J. Agric. Food Chem., 54, 2314-2319.

Search for more literature for 3.2.1.54

Application

Application	Comment	Organism
synthesis	CDase I-5 is applied to modify the starch structure to produce low-amylose starch products by incubating rice starch with this enzyme. The amylose content of rice starch decreases from 28.5 to 9% while the amylopectin content remains almost constant with no significant change in side chain length distribution	Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	-	Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
64000	-	12 * 64000, assembly of six dimers, SDS-PAGE	Bacillus sp. (in: Bacteria)

Organism

Organism	UniProt	Comment	Textmining
Bacillus sp. (in: Bacteria)	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
amylopectin + H2O	-	Bacillus sp. (in: Bacteria)	?	-	?
amylose + H2O	CDase I-5 shows high preference towards amylose	Bacillus sp. (in: Bacteria)	maltose + glucose	major hydrolysis products	?
cyclomaltoheptaose + H2O	-	Bacillus sp. (in: Bacteria)	?	-	?

Subunits

Subunits	Comment	Organism
dodecamer	12 * 64000, assembly of six dimers, SDS-PAGE	Bacillus sp. (in: Bacteria)

Synonyms

Synonyms	Comment	Organism
CDase I-5	-	Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	substrate: cyclomaltoheptaose	Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
30	60	30°C: about 45% of maximal activity, 60°C: about 80% of maximal activity, substrate: cyclomaltoheptaose	Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.19	-	amylopectin	-	Bacillus sp. (in: Bacteria)
2	8	amylose	-	Bacillus sp. (in: Bacteria)
3	-	cyclomaltoheptaose	-	Bacillus sp. (in: Bacteria)
22.11	-	amylose	-	Bacillus sp. (in: Bacteria)
50.36	-	amylopectin	-	Bacillus sp. (in: Bacteria)
78.18	-	cyclomaltoheptaose	-	Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	substrate: cyclomaltoheptaose	Bacillus sp. (in: Bacteria)

pH Range

pH Minimum	pH Maximum	Comment	Organism
6.5	8.5	pH 6.5: about 50% of maximal activity, pH 8.5: about 35% of maximal activity, substrate: cyclomaltoheptaose	Bacillus sp. (in: Bacteria)

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Release 2023.1 (January 2023)