Literature summary for 3.2.1.54 extracted from

Lee, H.S.; Kim, J.S.; Shim, K.; Kim, J.W.; Inouye, K.; Oneda, H.; Kim, Y.W.; Cheong, K.A.; Cha, H.; Woo, E.J.; Auh, J.H.; Lee, S.J.; Kim, J.W.; Park, K.H.
Dissociation/association properties of a dodecameric cyclomaltodextrinase. Effects of pH and salt concentration on the oligomeric state (2006), FEBS J., 273, 109-121.

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Protein Variants

Protein Variants	Comment	Organism
H49V/H539V	the mutant enzyme dissociates into dimers faster than the wild-type enzyme at both pH 6.0 and 7.0	Bacillus sp. (in: Bacteria)
H49V/H89V/H539V	the mutant enzyme dissociates into dimers faster than the wild-type enzyme at both pH 6.0 and 7.0	Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.454	-	beta-cyclodextrin	pH 7.0	Bacillus sp. (in: Bacteria)
0.889	-	beta-cyclodextrin	pH 6.0	Bacillus sp. (in: Bacteria)

Organism

Organism	UniProt	Comment	Textmining
Bacillus sp. (in: Bacteria)	-	I-5	-
Bacillus sp. (in: Bacteria) I-5	-	I-5	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
beta-cyclodextrin + H2O	-	Bacillus sp. (in: Bacteria)	?	-	?
beta-cyclodextrin + H2O	-	Bacillus sp. (in: Bacteria) I-5	?	-	?

Subunits

Subunits	Comment	Organism
dodecamer	exists exclusively as dodecamer at pH 7.0, forming an assembly of six 3D domain-swapped dimeric subunit. At pH 5.0-6.0 the enzyme is present as a dimer. Above pH 6.5 the dodecameric form is predominant. No dissociation of the dodecamer at pH 7.0 and above. Dissociation of wild-type CDase I-5 proceeds very fast at pH 7.0 in presence of 0.2-1.0 M of KCl. The mutant enzyme H49V/H89V/H539V dissociates into dimers faster than the wild-type enzyme at both pH 6.0 and 7.0	Bacillus sp. (in: Bacteria)

Synonyms

Synonyms	Comment	Organism
CDase I-5	-	Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3	-	beta-cyclodextrin	pH 7.0	Bacillus sp. (in: Bacteria)
8.5	-	beta-cyclodextrin	pH 6.0	Bacillus sp. (in: Bacteria)
78.2	-	beta-cyclodextrin	pH 7.0	Bacillus sp. (in: Bacteria)

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Release 2023.1 (January 2023)