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Literature summary for 3.2.1.54 extracted from

  • Hashimoto, Y.; Yamamoto, T.; Fujiwara, S.; Takagi, M.; Imanaka, T.
    Extracellular synthesis, specific recognition, and intracellular degradation of cyclomaltodextrins by the hyperthermophilic archaeon Thermococcus sp. strain B1001 (2001), J. Bacteriol., 183, 5050-5057.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.54

Cloned(Commentary)

Cloned (Comment) Organism
gene cgtB, DNA and amino acid sequence determination and analysis, expression in Escherichia coli BL21(DE3) Thermococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
79000
-
 x * 79000, recombinant enzyme, SDS-PAGE Thermococcus sp.

Organism

Organism UniProt Comment Textmining
Thermococcus sp.
-
-
-
Thermococcus sp. B1001
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli by heat denaturation Thermococcus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
640
-
 purified recombinant enzyme, substrate gamma-cyclomaltodextrin Thermococcus sp.
1080
-
 purified recombinant enzyme, substrate alpha-cyclomaltodextrin Thermococcus sp.
1940
-
 purified recombinant enzyme, substrate beta-cyclomaltodextrin Thermococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-cyclomaltodextrin + H2O 55% of the activity with beta-cyclomaltodextrin Thermococcus sp. alpha-D-glucose + maltose
-
 ?
alpha-cyclomaltodextrin + H2O 55% of the activity with beta-cyclomaltodextrin Thermococcus sp. B1001 alpha-D-glucose + maltose
-
 ?
beta-cyclomaltodextrin + H2O best substrate Thermococcus sp. alpha-D-glucose + maltose
-
 ?
beta-cyclomaltodextrin + H2O best substrate Thermococcus sp. B1001 alpha-D-glucose + maltose
-
 ?
gamma-cyclomaltodextrin + H2O 33% of the activity with beta-cyclomaltodextrin Thermococcus sp. alpha-D-glucose + maltose
-
 ?
gamma-cyclomaltodextrin + H2O 33% of the activity with beta-cyclomaltodextrin Thermococcus sp. B1001 alpha-D-glucose + maltose
-
 ?
linear maltodextrins + H2O with a small glucose unit, low activity Thermococcus sp. alpha-D-glucose + maltose
-
 ?
linear maltodextrins + H2O with a small glucose unit, low activity Thermococcus sp. B1001 alpha-D-glucose + maltose
-
 ?
additional information no activity with pullulan and maltose, soluble starch is a poor substrate Thermococcus sp. ?
-
 ?
additional information no activity with pullulan and maltose, soluble starch is a poor substrate Thermococcus sp. B1001 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 79000, recombinant enzyme, SDS-PAGE Thermococcus sp.

Synonyms

Synonyms Comment Organism
CDase
-
 Thermococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
 recombinant enzyme Thermococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 recombinant enzyme Thermococcus sp.