Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.54 extracted from

  • Lee, H.S.; Kim, M.S.; Cho, H.S.; Kim, J.I.; Kim, T.J.; Choi, J.H.; Park, C.; Oh, B.H.; Park, K.H.
    Cyclomaltodextrinase, neopullulanase, and maltogenic amylase are nearly indistinguishable from each other (2002), J. Biol. Chem., 277, 21891-21897.
    View publication on PubMed
Search for more literature for 3.2.1.54

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Escherichia coli strain MC1061 Bacillus sp. (in: Bacteria)

Crystallization (Commentary)

Crystallization (Comment) Organism
10 mg/ml purified recombinant enzyme in 10 mM HEPES, pH 7.5 and 1 mM 2-mercaptoethanol, hanging drop vapour diffusion method by addition of equal amount of 0.002 ml of precipitant solution containing 50% PEG 200, and 0.1 M HEPES, pH 7.0, equilibration against 1 ml, 22°C, X-ray diffraction structure determination and analysis at 3.3 A resolution Bacillus sp. (in: Bacteria)

Protein Variants

Protein Variants Comment Organism
additional information construction of a mutant enzyme lacking the C-terminal domain, expression as insoluble protein in Escherichia coli Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.43
-
 beta-cyclomaltodextrin pH 6.0, dimer Bacillus sp. (in: Bacteria)
8.68
-
 beta-cyclomaltodextrin pH 7.0, dodecamer Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
 alcalophilic strain I-5
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli by ammonium sulfate precipitation and Sepharose Q chromatography Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-cyclomaltodextrin + H2O
-
 Bacillus sp. (in: Bacteria) alpha-D-glucose + maltose
-
 ?
beta-cyclomaltodextrin + H2O
-
 Bacillus sp. (in: Bacteria) alpha-D-glucose + maltose
-
 ?
gamma-cyclomaltodextrin + H2O
-
 Bacillus sp. (in: Bacteria) alpha-D-glucose + maltose
-
 ?
pullulan + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
starch + H2O
-
 Bacillus sp. (in: Bacteria) alpha-D-glucose + maltose
-
 ?

Subunits

Subunits Comment Organism
dimer domain-swapped dimer Bacillus sp. (in: Bacteria)
dodecamer dodecameric assembly of 6 copies of the dimer, most active form Bacillus sp. (in: Bacteria)
More pH-dependent association and dissociation of the enzyme dimeric units, structure determination and analysis, enzyme has a beta-stranded N-terminal domain, residues 1-123, a central (alphabeta)8 barrel domain, and a beta-stranded C-terminal domain, residues 505-583 Bacillus sp. (in: Bacteria)

Synonyms

Synonyms Comment Organism
CDase
-
 Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.7
-
 beta-cyclomaltodextrin pH 7.0, dodecamer Bacillus sp. (in: Bacteria)
1.68
-
 beta-cyclomaltodextrin pH 6.0, dimer Bacillus sp. (in: Bacteria)