Cloned (Comment) | Organism |
---|---|
plasmid pPG22, expression in Escherichia coli | Thermoanaerobacter thermohydrosulfuricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | - |
Thermoanaerobacter thermohydrosulfuricus | |
Cu2+ | - |
Thermoanaerobacter thermohydrosulfuricus | |
Hg2+ | - |
Thermoanaerobacter thermohydrosulfuricus | |
N-bromosuccinimide | - |
Thermoanaerobacter thermohydrosulfuricus | |
p-chloromercuribenzoate | - |
Thermoanaerobacter thermohydrosulfuricus | |
Zn2+ | - |
Thermoanaerobacter thermohydrosulfuricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
gamma-cyclodextrin | - |
Thermoanaerobacter thermohydrosulfuricus | |
2.1 | - |
beta-cyclodextrin | - |
Thermoanaerobacter thermohydrosulfuricus | |
2.5 | - |
alpha-cyclodextrin | - |
Thermoanaerobacter thermohydrosulfuricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
monomer SDS-PAGE | Thermoanaerobacter thermohydrosulfuricus |
68030 | - |
denatured protein estimated by SDS-PAGE, native enzyme determined by gel filtration | Thermoanaerobacter thermohydrosulfuricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoanaerobacter thermohydrosulfuricus | - |
39E | - |
Thermoanaerobacter thermohydrosulfuricus 39E | - |
39E | - |
Purification (Comment) | Organism |
---|---|
gene encoding enzyme cloned in Escherichia coli, expressed and purified 12fold from Escherichia coli | Thermoanaerobacter thermohydrosulfuricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
62 | - |
gel filtration | Thermoanaerobacter thermohydrosulfuricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-cyclodextrin + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | maltose + D-glucose | - |
? | |
amylopectin + H2O | rate of hydrolysis less than 5% of the rate of alpha-cyclodextrin hydrolysis | Thermoanaerobacter thermohydrosulfuricus | maltose + ? | - |
? | |
amylose + H2O | less than 5% of the rate of alpha-cyclodextrin hydrolysis | Thermoanaerobacter thermohydrosulfuricus | ? | - |
? | |
beta-cyclodextrin + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | glucose + maltose + long chain linear oligomers | - |
? | |
beta-cyclodextrin + H2O | - |
Thermoanaerobacter thermohydrosulfuricus 39E | glucose + maltose + long chain linear oligomers | - |
? | |
cyclomaltodextrin + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | maltodextrin | - |
? | |
cyclomaltodextrin + H2O | - |
Thermoanaerobacter thermohydrosulfuricus 39E | maltodextrin | - |
? | |
gamma-cyclodextrin + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | maltose + D-glucose | - |
? | |
glycogen + H2O | relative rate of hydrolysis 0,1% | Thermoanaerobacter thermohydrosulfuricus | ? | - |
? | |
maltodextrins + H2O | linear, enzyme degrades the linear molecules by splitting off maltose units from the nonreducing end of the chain | Thermoanaerobacter thermohydrosulfuricus | maltose + glucose | - |
? | |
maltodextrins + H2O | linear, enzyme degrades the linear molecules by splitting off maltose units from the nonreducing end of the chain | Thermoanaerobacter thermohydrosulfuricus 39E | maltose + glucose | - |
? | |
maltoheptaose + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | D-glucose + maltose + ? | - |
? | |
maltohexaose + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | maltotetraose + maltose | - |
? | |
maltopentaose + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | maltose + maltotriose | - |
? | |
maltotetraose + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | 2 maltose | - |
? | |
maltotriose + H2O | slowly | Thermoanaerobacter thermohydrosulfuricus | ? | - |
? | |
maltotriose + H2O | slowly | Thermoanaerobacter thermohydrosulfuricus 39E | ? | - |
? | |
additional information | not: maltose | Thermoanaerobacter thermohydrosulfuricus | ? | - |
? | |
additional information | not: maltose | Thermoanaerobacter thermohydrosulfuricus 39E | ? | - |
? | |
pullulan + H2O | rate of hydrolysis less than 5% of the rate of alpha-cyclodextrin hydrolysis | Thermoanaerobacter thermohydrosulfuricus | glucose + panose + isopanose + large saccharides containing alpha 1,6-glucosidic bonds | - |
? | |
starch + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | glucose + maltose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
thermophilic CDase | - |
Thermoanaerobacter thermohydrosulfuricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
beta-cyclodextrin as substrate | Thermoanaerobacter thermohydrosulfuricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 75 | - |
Thermoanaerobacter thermohydrosulfuricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
incubation for 1 h led to a 15% loss of activity | Thermoanaerobacter thermohydrosulfuricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.9 | - |
- |
Thermoanaerobacter thermohydrosulfuricus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | - |
Thermoanaerobacter thermohydrosulfuricus |