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Literature summary for 3.2.1.54 extracted from
- Purification and properties of the cyclodextrinase of Bacillus macerans (1968), Biochemistry, 7, 121-125.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.62 | - |
alpha-cyclodextrin | - |
Paenibacillus macerans |  |
| 2.65 | - |
beta-cyclodextrin | - |
Paenibacillus macerans | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cyclomaltodextrins + H2O | Paenibacillus macerans | - |
linear maltodextrins | - |
? | |
| cyclomaltodextrins + H2O | Paenibacillus macerans ATCC 8514 | - |
linear maltodextrins | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paenibacillus macerans | - |
ATCC8514 | - |
| Paenibacillus macerans ATCC 8514 | - |
ATCC8514 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified 40fold, highly purified, not completely free from contaminations | Paenibacillus macerans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Paenibacillus macerans |
| 5 | - |
crude enzyme, purified 1fold | Paenibacillus macerans |
| 175 | - |
- |
Paenibacillus macerans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-cyclomaltodextrin + H2O | - |
Paenibacillus macerans | alpha-D-glucose + maltose | - |
? | |
| alpha-cyclomaltodextrin + H2O | - |
Paenibacillus macerans ATCC 8514 | alpha-D-glucose + maltose | - |
? | |
| cyclomaltodextrin + H2O | - |
Paenibacillus macerans | maltodextrin | - |
ir | |
| cyclomaltodextrin + H2O | - |
Paenibacillus macerans ATCC 8514 | maltodextrin | - |
ir | |
| cyclomaltodextrins + H2O | - |
Paenibacillus macerans | linear maltodextrins | - |
? | |
| cyclomaltodextrins + H2O | - |
Paenibacillus macerans ATCC 8514 | linear maltodextrins | - |
? | |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 55 | - |
Paenibacillus macerans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | 6.4 | - |
Paenibacillus macerans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.6 | - |
Paenibacillus macerans |
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