Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.54 extracted from

  • Kitahata, S.; Taniguchi, M.; Beltran, S.D.; Sugimoto, T.; Okada, S.
    Purification and some properties of cyclodextrinase from Bacillus coagulans (1983), Agric. Biol. Chem., 47, 1441-1447.
No PubMed abstract available
Search for more literature for 3.2.1.54

Inhibitors

Inhibitors Comment Organism Structure
Fe2+ reduces activity to79% Weizmannia coagulans
Fe3+ reduces activity to 50% Weizmannia coagulans
Hg2+ reduces activity to 24% Weizmannia coagulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.47
-
 gamma-cyclodextrin
-
 Weizmannia coagulans
1.4
-
 maltohexaose
-
 Weizmannia coagulans
2.3
-
 maltopentaose
-
 Weizmannia coagulans
2.8
-
 beta-cyclodextrin
-
 Weizmannia coagulans
4
-
 maltotetraose
-
 Weizmannia coagulans
4.5
-
 maltotriose
-
 Weizmannia coagulans
10
-
 alpha-cyclodextrin
-
 Weizmannia coagulans

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
 Weizmannia coagulans 5622
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ increase activity to 112%/102% Weizmannia coagulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 SDS-PAGE Weizmannia coagulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cyclomaltodextrins + H2O Weizmannia coagulans
-
 linear maltodextrins
-
 ?

Organism

Organism UniProt Comment Textmining
Weizmannia coagulans
-
 gram positive bacterium, isolated from soil
-

Purification (Commentary)

Purification (Comment) Organism
not completely purified, minor contamination in ultracentrifugation Weizmannia coagulans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Weizmannia coagulans
0.12
-
 crude enzyme Weizmannia coagulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclomaltodextrin + H2O alpha-, beta- and gamma Weizmannia coagulans maltodextrin
-
 ?
cyclomaltodextrins + H2O
-
 Weizmannia coagulans linear maltodextrins
-
 ?
maltodextrins + H2O
-
 Weizmannia coagulans maltose + glucose
-
 ?
maltohexaose + H2O
-
 Weizmannia coagulans maltotetraose + maltose
-
 ?
maltopentaose + H2O
-
 Weizmannia coagulans maltose + maltotriose
-
 ?
maltotetraose + H2O
-
 Weizmannia coagulans 2 maltose
-
 ?
maltotriose + H2O slowly Weizmannia coagulans ?
-
 ?
additional information slowly: short chain amylose Weizmannia coagulans ?
-
 ?
additional information not: maltose Weizmannia coagulans ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Weizmannia coagulans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 stable up at pH 6.0-7.3 on 2 h incubation Weizmannia coagulans
45
-
 stable at temperatures below , pH 7 Weizmannia coagulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
-
 Weizmannia coagulans

pH Range

pH Minimum pH Maximum Comment Organism
5 8
-
 Weizmannia coagulans

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 7.3 40°C, 2 h stable Weizmannia coagulans