Application | Comment | Organism |
---|---|---|
analysis | comparison of glycolytic and chitinolytic enzyme activities between desert and oasis flies of Phlebotomus papatasi to evaluate potential differences in susceptibility to infection with Leishmania major | Phlebotomus papatasi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
mannose | inhibition of beta-N-acetylgalactosaminidase but activation of alpha-N-acetylgalactosaminidase, three concentrations of 10, 50, and 100 mM tested | Phlebotomus papatasi | |
N-acetylgalactosamine | dose dependent inhibition, at lower levels than inhibition by N-acetylglucosamine, three concentrations of 10, 50, and 100 mM tested | Phlebotomus papatasi | |
N-acetylglucosamine | constant inhibition, three concentrations of 10, 50, and 100 mM tested | Phlebotomus papatasi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phlebotomus papatasi | - |
desert and oasis flies studied, male and female, Neot Hakikar (oasis), Jordan Valley spring (wet), Kfar Adumim starved (arid), Jordan Valley autumn (arid) | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
whole body | whole fly homogenates | Phlebotomus papatasi | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
assay described, enzyme activity measured in homogenates of 10 to 15 male and female flies before and after sucrose meals, monitoring by spectroscopy, values of relative activity indicated | Phlebotomus papatasi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl-N-acetyl-beta-D-galactosaminide + H2O | activity measured in whole fly homogenates, substrate concentration of 6 mM in citrate buffer, flies of arid regions tend to produce more beta-N-acetylgalactosaminidase than those originating in sugar-rich environments | Phlebotomus papatasi | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-N-acetylgalactosaminidase | - |
Phlebotomus papatasi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | - |
assay at, pH-optimum estimated for | Phlebotomus papatasi |