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Literature summary for 3.2.1.53 extracted from
- Isolation of beta-N-acetylhexosaminidase, beta-N-acetylglucosaminidase, and beta-N-acetylgalactosaminidase from calf brain (1967), Biochemistry, 6, 2775-2782.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| N-ethylmaleimide | 1mM solution increase the reaction rates 2-3fold | Bos taurus |  |
General Stability
| General Stability | Organism |
|---|---|
| cysteine, stabilization | Bos taurus |
| dithiothreitol, stabilization | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AgNO3 | - |
Bos taurus | |
| N-acetylgalactosamine | competitive inhibition | Bos taurus | |
| N-acetylglucosamine | no inhibition at N-acetylglucosamine concentration of 40 mM | Bos taurus | |
| p-hydroxymercuribenzoate | - |
Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.35 | - |
4-nitrophenyl beta-acetylgalactosaminide | - |
Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-beta-D-galactosaminides + H2O | Bos taurus | - |
? | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Acetone | inactivation | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
calf | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Bos taurus | - |
| liver | - |
Bos taurus | - |
| spleen | - |
Bos taurus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20 ºC, inactivation after several weeks | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-acetylgalactosaminide + H2O | - |
Bos taurus | 4-nitrophenol + beta-acetylgalactosamine | - |
? | |
| beta-phenyl-N-acetylgalactosamines + H2O | substrate specificity 85% | Bos taurus | ? | - |
? | |
| N-acetyl-beta-D-galactosaminides + H2O | e.g. 4-methylumbelliferyl-beta-N-acetylgalactosaminide | Bos taurus | N-acetyl-D-galactosamine + an alcohol | e.g. 4-methylumbelliferone | ? | |
| N-acetyl-beta-D-galactosaminides + H2O | - |
Bos taurus | ? | - |
? | |
| p-nitrophenyl beta-N-acetylgalactosaminide + H2O | - |
Bos taurus | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
p-nitrophenyl beta-N-acetylgalactosaminide | Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
not active below, p-nitrophenyl beta-N-acetylgalactosaminide | Bos taurus |
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