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Literature summary for 3.2.1.50 extracted from

  • Mersman, G.; von Figura, K.; Buddecke, E.
    Physical properties and biological activities of two forms of alpha-N-acetylglucosaminidase from bovine spleen (1974), Biochim. Biophys. Acta, 364, 88-96.
    View publication on PubMed
Search for more literature for 3.2.1.50

General Stability

General Stability Organism
entire inactivation by gel electrophoresis in the presence of sodium dodecylsulfate Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043 0.61 2-nitrophenyl alpha-N-acetylglucosaminide form I 0.067, form II 0.043 Bos taurus
0.12 1.6 phenyl-alpha-N-acetylglucosaminide form I 0.32, form II 0.29 Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Hg2+ complete inactivation at 20 mM HgCl2 Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64500
-
 form II after gel filtration or disc electrophoresis Bos taurus
127500
-
 form I after gel filtration or disc electrophoresis Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(GlcNAcalpha(1-4))n + H2O Bos taurus involved in the degradation of heparin and heparan sulfate (GlcNAcalpha(1-4))n-1 + 2-(acetylamino)-2-deoxy-beta-D-glucopyranose
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
spleen
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0075
-
 purified enzyme Bos taurus

Storage Stability

Storage Stability Organism
37°C, 4 days, no loss of activity, but complete dissociation of form I into form II Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(GlcNAcalpha(1-4))n + H2O involved in the degradation of heparin and heparan sulfate Bos taurus (GlcNAcalpha(1-4))n-1 + 2-(acetylamino)-2-deoxy-beta-D-glucopyranose
-
 ?
2-nitrophenyl alpha-N-acetylglucosaminide + H2O
-
 Bos taurus 2-nitrophenol + alpha-N-acetylglucosamine
-
 ?
phenyl-N-acetyl-alpha-D-glucosaminide + H2O
-
 Bos taurus phenol + N-acetyl-alpha-D-glucosamine
-
 ?

Synonyms

Synonyms Comment Organism
2-acetamido-2-deoxy-alpha-D-glucoside acetamidodeoxyglucohydrolase
-
 Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 70 in citrate buffer, pH 6.0, no inactivation by 50°C heating, 90% inactivation at 70°C for 30 min Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.7
-
 phenyl-alpha-N-acetylglucosaminide Bos taurus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10.5 rapid loss of activity above and below Bos taurus