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Literature summary for 3.2.1.50 extracted from

  • von Figura, K.
    Human alpha-N-acetylglucosaminidase. 1. Purification and properties (1977), Eur. J. Biochem., 80, 525-533.
No PubMed abstract available
Search for more literature for 3.2.1.50

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin stabilization and acceleration of activity betweeen 0.05-0.1%, 2-3fold activity at 0.025% Homo sapiens

General Stability

General Stability Organism
no proteolysis with pronase, bromelain and trypsin, 15% loss of activity after 30 min incubation with papain Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
dermatan sulfate competitive inhibition at 1 mM with p-nitrophenyl-alpha-N-acetylglucosaminide and UDP-N-acetylglucosamine as substrates Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.39
-
 UDP-N-acetylglucosamine 0.58 in the presence of 0.025% bovine serum albumin Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ complete inhibition at 10 mM Homo sapiens
Hg2+ complete inhibition at 10 mM Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
300000 307000 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(GlcNAcalpha(1-4))n + H2O Homo sapiens
-
 (GlcNAcalpha(1-4))n-1 + 2-(acetylamino)-2-deoxy-beta-D-glucopyranose
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
urine
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.115 1.2 purified enzyme, p-nitrophenyl-N-acetyl-alpha-D-glucosaminide as substrate Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(GlcNAcalpha(1-4))n + H2O
-
 Homo sapiens (GlcNAcalpha(1-4))n-1 + 2-(acetylamino)-2-deoxy-beta-D-glucopyranose
-
 ?
o-nitrophenyl-N-acetyl-alpha-D-glucosaminide + H2O
-
 Homo sapiens o-nitrophenol + N-acetyl-alpha-D-glucosamine
-
 ?
p-nitrophenyl-N-acetyl-alpha-D-glucosaminide + H2O
-
 Homo sapiens p-nitrophenol + N-acetyl-alpha-D-glucosamine
-
 ?
phenyl-N-acetyl-alpha-D-glucosaminide + H2O
-
 Homo sapiens phenol + N-acetyl-alpha-D-glucosamine
-
 ?
UDP-N-acetyl-alpha-D-glucosamine + H2O
-
 Homo sapiens uridine-5'-diphosphate + N-acetyl-alpha-D-glucosamine
-
 ?
uridine-5'-diphospho-N-acetyl-alpha-D-glucosamine
-
 Homo sapiens uridine-5'-diphosphate + N-acetyl-alpha-D-glucosamine
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 70 90% loss of activity after heating at 60°C for 10 min in the presence of 0.5% bovine serum albumin Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2
-
 UDP-N-acetylglucosamine Homo sapiens
4.6
-
 phenyl-alpha-N-acetylglucosaminide Homo sapiens
4.8
-
 p-nitrophenyl-alpha-N-acetylglucosaminide Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9
-
 Homo sapiens