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Literature summary for 3.2.1.50 extracted from

  • Roehrborn, W.; von Figura, K.
    Human placenta alpha-N-acetylglucosaminidase: purification, characterization and demonstration of multiple recognition forms (1978), Hoppe-Seyler's Z. Physiol. Chem., 359, 1353-1362.
    View publication on PubMed
Search for more literature for 3.2.1.50

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin stabilization and acceleration of activity betweeen 0.05-0.1%, 2-3fold activity at 0.025% Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043 0.61 o-nitrophenyl-alpha-N-acetylglucosaminide 0.23-0.25 in the presence of 0.025% bovine serum albumin Homo sapiens
0.12 1.6 phenyl-alpha-N-acetylglucosaminide 0.46-0.66 in the presence of 0.025% bovine serum albumin Homo sapiens
0.13 0.2 p-nitrophenyl-alpha-N-acetylglucosaminide 0.21-0.55 in the presence of 0.025% bovine serum albumin Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
81000
-
 alpha, beta, 1 * 86500 + 1 * 81000, SDS-PAGE Homo sapiens
86500
-
 alpha, beta, 1 * 86500 + 1 * 81000, SDS-PAGE Homo sapiens
300000 307000 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(GlcNAcalpha(1-4))n + H2O Homo sapiens involved in the degradation of heparin and heparan sulfate (GlcNAcalpha(1-4))n-1 + 2-(acetylamino)-2-deoxy-beta-D-glucopyranose
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 23.4% carbohydrates, e.g. glucose, mannose, galactose Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
placenta
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.115 1.2 purified enzyme, p-nitrophenyl-N-acetyl-alpha-D-glucosaminide as substrate Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(GlcNAcalpha(1-4))n + H2O involved in the degradation of heparin and heparan sulfate Homo sapiens (GlcNAcalpha(1-4))n-1 + 2-(acetylamino)-2-deoxy-beta-D-glucopyranose
-
 ?
o-nitrophenyl-N-acetyl-alpha-D-glucosaminide + H2O
-
 Homo sapiens o-nitrophenol + N-acetyl-alpha-D-glucosamine
-
 ?
p-nitrophenyl-N-acetyl-alpha-D-glucosaminide + H2O
-
 Homo sapiens p-nitrophenol + N-acetyl-alpha-D-glucosamine
-
 ?
phenyl-N-acetyl-alpha-D-glucosaminide + H2O
-
 Homo sapiens phenol + N-acetyl-alpha-D-glucosamine
-
 ?
uridine-5'-diphospho-N-acetyl-alpha-D-glucosamine
-
 Homo sapiens uridine-5'-diphosphate + N-acetyl-alpha-D-glucosamine
-
 ?

Subunits

Subunits Comment Organism
dimer alpha, beta, 1 * 86500 + 1 * 81000, SDS-PAGE Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 70 no effect by 50°C heating, 50% inactivation after 12 min at 60°C and 2 min at 70°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4
-
 p-nitrophenyl-alpha-N-acetylglucosaminide Homo sapiens
4.5
-
 o-nitrophenyl-alpha-N-acetylglucosaminide Homo sapiens
4.8
-
 phenyl-alpha-N-acetylglucosaminide Homo sapiens
5.7
-
 sucrose-stabilized pH-gradient, after treatment with neuraminidase Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
4.3 6.5
-
 Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9 no loss of activity by dialysis against 100 mM NaCl for 16 h, rapid loss of activity below pH 4 and above pH 10 Homo sapiens