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Literature summary for 3.2.1.49 extracted from

  • Chakladar, S.; Shamsi Kazem Abadi, S.; Bennet, A.
    A mechanistic study on the alpha-N-acetylgalactosaminidase from E. meningosepticum: A family 109 glycoside hydrolase (2014), MedChemComm, 5, 1188-1192.
No PubMed abstract available
Search for more literature for 3.2.1.49

Organism

Organism UniProt Comment Textmining
Elizabethkingia meningoseptica
-
-
-

Reaction

Reaction Comment Organism Reaction ID
alpha-D-GalNAc-(1->3)-beta-D-GalNAc-(1->3)-alpha-D-Gal-(1->4)-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1)-ceramide + H2O = D-GalNAc + beta-D-GalNAc-(1->3)-alpha-D-Gal-(1->4)-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1)-ceramide E2 elimination following hydride transfer from C3 Elizabethkingia meningoseptica
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl N-acetyl-alpha-D-galactosamine + H2O
-
 Elizabethkingia meningoseptica 4-nitrophenol + N-acetyl-alpha-galactosamine
-
 ?
additional information the enzyme catalyses the hydrolysis of aryl 2-acetamido-2-deoxy-alpha-D-galactopyranosides Elizabethkingia meningoseptica ?
-
 ?

Synonyms

Synonyms Comment Organism
alpha-NAGAL
-
 Elizabethkingia meningoseptica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Elizabethkingia meningoseptica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
19.7
-
 4-nitrophenyl N-acetyl-alpha-D-galactosamine recombinant enzyme, pH 7.5, 37°C Elizabethkingia meningoseptica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Elizabethkingia meningoseptica

General Information

General Information Comment Organism
evolution the enzyme belongs to the glycoside hydrolase family GH 109 Elizabethkingia meningoseptica

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1250
-
 4-nitrophenyl N-acetyl-alpha-D-galactosamine recombinant enzyme, pH 7.5, 37°C Elizabethkingia meningoseptica