Cloned (Comment) | Organism |
---|---|
the gene encoding the clostridial enzyme is cloned in an Escherichia coli T7 expression system | Clostridium perfringens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
p-nitrophenyl-N-acetyl-alpha-D-galactosaminide | pH 6.5, 37°C, recombinant enzyme | Clostridium perfringens | |
1.62 | - |
o-nitrophenyl-N-acetyl-alpha-D-galactosaminide | pH 6.5, 37°C, recombinant enzyme | Clostridium perfringens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
719600 | - |
x * 719600, denatured recombinant enzyme, x * 724200, native recombinant enzyme, SDS-PAGE | Clostridium perfringens |
724200 | - |
x * 719600, denatured recombinant enzyme, x * 724200, native recombinant enzyme, SDS-PAGE | Clostridium perfringens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium perfringens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Clostridium perfringens |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
84.11 | - |
- |
Clostridium perfringens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is highly selective for terminal N-acetylgalactosamine residues, sugars other than N-acetyl-alpha-D-galactosaminide are no substrates | Clostridium perfringens | ? | - |
? | |
o-nitrophenyl N-acetyl-alpha-D-galactosaminide + H2O | substrate from the blood type A2 antigen producing H antigen, blood type O | Clostridium perfringens | o-nitrophenol + N-acetyl-alpha-D-galactosamine | - |
? | |
p-nitrophenyl-N-acetyl-alpha-D-galactosaminide + H2O | - |
Clostridium perfringens | p-nitrophenol + N-acetyl-alpha-D-galactosamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 719600, denatured recombinant enzyme, x * 724200, native recombinant enzyme, SDS-PAGE | Clostridium perfringens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 45 | - |
Clostridium perfringens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | recombinant enzyme | Clostridium perfringens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | - |
Clostridium perfringens |