Application | Comment | Organism |
---|---|---|
medicine | the enzyme can be employed as a diagnostic/prognostic tool for patients with salivary gland adenocarcima | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
x * 48000, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
enzyme from salivary gland cells | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
alpha-D-GalNAc-(1->3)-beta-D-GalNAc-(1->3)-alpha-D-Gal-(1->4)-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1)-ceramide + H2O = D-GalNAc + beta-D-GalNAc-(1->3)-alpha-D-Gal-(1->4)-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1)-ceramide | the enzyme exhibits an endo-enzyme activity in addition to an exo-activity | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
carcinoma cell | high levels, salivary gland and gingival | Homo sapiens | - |
embryo | - |
Homo sapiens | - |
fibroblast | low levels, embryonic lung and gingival | Homo sapiens | - |
HEL cell | 299, low levels | Homo sapiens | - |
keratinocyte | low levels | Homo sapiens | - |
lung | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0002 | - |
cell extract of gingival keratinocyte | Homo sapiens |
0.0003 | - |
cell extract of gingival fibroblast | Homo sapiens |
0.0004 | - |
cell extract of embryonic lung fibroblast | Homo sapiens |
0.005 | - |
cell extract of gingival carcinoma | Homo sapiens |
0.012 | - |
cell extract of salivary gland carcinoma | Homo sapiens |
2279 | - |
purified fraction from salivary gland carcinoma | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme inactivates the macrophage activing factor produced from Gc protein and this results in reduced phagocytic activity and superoxide-generation capacity of monocytes/macrophages | Homo sapiens | ? | - |
? | |
p-nitrophenyl 2-acetamide-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside + H2O | - |
Homo sapiens | p-nitrophenol + 2-acetamide-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside | - |
? | |
p-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Homo sapiens | p-nitrophenol + alpha-D-galactopyranose | - |
? | |
p-nitrophenyl N-acetyl-alpha-D-galactosaminide + H2O | - |
Homo sapiens | p-nitrophenol + N-acetyl-alpha-D-galactosamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 48000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
alpha-NaGalase | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
stable for 6 h in 0.05 sodium citrate buffer, pH 4.2 | Homo sapiens |
40 | - |
40% loss of activity after 30 min | Homo sapiens |
45 | - |
55% loss of activity after 10 min | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | 6.8 | - |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | below pH 3.6 the activity declines rapidly and at pH 3.0 retains about 1% of the orginal activity, above pH 7.5 the activity declines rapidly and at pH 8.0 retains about 4% of the orginal activity | Homo sapiens |