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Literature summary for 3.2.1.49 extracted from

  • Matsuura, T.; Uematsu, T.; Yamaoka, M.; Furusawa, K.
    Effect of salivary gland adenocarcinoma cell-derived alpha-N-acetylgalactosaminidase on the bioactivity of macrophage activating factor (2004), Int. J. Oncol., 24, 521-528.
    View publication on PubMed

Application

Application Comment Organism
medicine the enzyme can be employed as a diagnostic/prognostic tool for patients with salivary gland adenocarcima Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
x * 48000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
enzyme from salivary gland cells Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
alpha-D-GalNAc-(1->3)-beta-D-GalNAc-(1->3)-alpha-D-Gal-(1->4)-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1)-ceramide + H2O = D-GalNAc + beta-D-GalNAc-(1->3)-alpha-D-Gal-(1->4)-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1)-ceramide the enzyme exhibits an endo-enzyme activity in addition to an exo-activity Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
carcinoma cell high levels, salivary gland and gingival Homo sapiens
-
embryo
-
Homo sapiens
-
fibroblast low levels, embryonic lung and gingival Homo sapiens
-
HEL cell 299, low levels Homo sapiens
-
keratinocyte low levels Homo sapiens
-
lung
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0002
-
cell extract of gingival keratinocyte Homo sapiens
0.0003
-
cell extract of gingival fibroblast Homo sapiens
0.0004
-
cell extract of embryonic lung fibroblast Homo sapiens
0.005
-
cell extract of gingival carcinoma Homo sapiens
0.012
-
cell extract of salivary gland carcinoma Homo sapiens
2279
-
purified fraction from salivary gland carcinoma Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme inactivates the macrophage activing factor produced from Gc protein and this results in reduced phagocytic activity and superoxide-generation capacity of monocytes/macrophages Homo sapiens ?
-
?
p-nitrophenyl 2-acetamide-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside + H2O
-
Homo sapiens p-nitrophenol + 2-acetamide-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
-
Homo sapiens p-nitrophenol + alpha-D-galactopyranose
-
?
p-nitrophenyl N-acetyl-alpha-D-galactosaminide + H2O
-
Homo sapiens p-nitrophenol + N-acetyl-alpha-D-galactosamine
-
?

Subunits

Subunits Comment Organism
? x * 48000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
alpha-NaGalase
-
Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
stable for 6 h in 0.05 sodium citrate buffer, pH 4.2 Homo sapiens
40
-
40% loss of activity after 30 min Homo sapiens
45
-
55% loss of activity after 10 min Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 6.8
-
Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
3 8 below pH 3.6 the activity declines rapidly and at pH 3.0 retains about 1% of the orginal activity, above pH 7.5 the activity declines rapidly and at pH 8.0 retains about 4% of the orginal activity Homo sapiens