Activating Compound | Comment | Organism | Structure |
---|---|---|---|
maltose | - |
Bacillus subtilis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, the gene coding the enzyme has been identified in the ORF yvdL/malL | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.135 | - |
maltose | - |
Bacillus subtilis | |
0.21 | - |
p-nitrophenyl-alpha-D-glucoside | - |
Bacillus subtilis | |
0.455 | - |
isomaltose | - |
Bacillus subtilis | |
10.2 | - |
sucrose | - |
Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltose + H2O | Bacillus subtilis | - |
2 glucose | - |
? | |
sucrose + H2O | Bacillus subtilis | - |
D-glucose + D-fructose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
maltose-inducible alpha-glucosidase MalL, sucrase-isomaltase-maltase | - |
Purification (Comment) | Organism |
---|---|
produced and purified in Escherichia coli RB791/pMalL | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.47 | - |
maltose | Bacillus subtilis |
2.02 | - |
isomaltose | Bacillus subtilis |
4.47 | - |
sucrose | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isomaltose + H2O | - |
Bacillus subtilis | 2 D-glucose | - |
? | |
maltose + H2O | - |
Bacillus subtilis | 2 glucose | - |
? | |
maltose + H2O | - |
Bacillus subtilis | 2 D-glucose | - |
? | |
p-nitrophenyl-alpha-glucoside + H2O | - |
Bacillus subtilis | p-nitrophenol + D-glucose | - |
? | |
sucrose + H2O | - |
Bacillus subtilis | D-glucose + D-fructose | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
with PNPG as substrate, at pH 5.75 and 8.0 activity reduced to 50% | Bacillus subtilis |