Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.41 extracted from

  • Siroosi, M.; Amoozegar, M.A.; Khajeh, K.; Fazeli, M.; Rezaei, M.H.
    Purification and characterization of a novel extracellular halophilic and organic solvent-tolerant amylopullulanase from the haloarchaeon, Halorubrum sp. strain Ha25 (2014), Extremophiles, 18, 25-33.
    View publication on PubMed
Search for more literature for 3.2.1.41

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 soluble starch Km-value: 1.8 mg/ml Halorubrum sp. Ha25
additional information
-
 pullulan KM-value: 4 mg/ml Halorubrum sp. Ha25

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Halorubrum sp. Ha25
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl the enzyme is active over a wide range of concentrations (0–4.5 M) of NaCl Halorubrum sp. Ha25

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14000
-
 x * 14000, SDS-PAGE Halorubrum sp. Ha25

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone 20%, half-life of pullulytic activity: 155 h, half-life of amylolytic activity: 255 h Halorubrum sp. Ha25
chloroform 20%, half-life of pullulytic activity: 150 h, half-life of amylolytic activity: 230 h Halorubrum sp. Ha25
dimethyl sulfoxide 20%, half-life of pullulytic activity: 46 h, half-life of amylolytic activity: 41 h Halorubrum sp. Ha25
Ethanol 20%, half-life of pullulytic activity: 57 h, half-life of amylolytic activity: 99 h Halorubrum sp. Ha25
additional information the enzyme is more stable in the presence of non-polar organic solvents than polar solvents (20%) Halorubrum sp. Ha25
N,N-dimethylformamide 20%, half-life of pullulytic activity: 65 h, half-life of amylolytic activity: 101 h Halorubrum sp. Ha25
n-Butanol 20%, half-life of pullulytic activity: 125 h, half-life of amylolytic activity: 215 h Halorubrum sp. Ha25
propanol 20%, half-life of pullulytic activity: 33 h, half-life of amylolytic activity: 53 h Halorubrum sp. Ha25

Organism

Organism UniProt Comment Textmining
Halorubrum sp. Ha25
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Halorubrum sp. Ha25

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pullulan + H2O
-
 Halorubrum sp. Ha25 ?
-
 ?
soluble starch + H2O
-
 Halorubrum sp. Ha25 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 14000, SDS-PAGE Halorubrum sp. Ha25

Synonyms

Synonyms Comment Organism
amylopullulanase
-
 Halorubrum sp. Ha25

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Halorubrum sp. Ha25

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 65 40°C: about 45% of maximal activity, 65°C: about 55% of maximal activity Halorubrum sp. Ha25

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 half-life: 155 min Halorubrum sp. Ha25
60
-
 half-life: 43 min Halorubrum sp. Ha25
70
-
 half-life: 21 min Halorubrum sp. Ha25

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 amylolytic activity Halorubrum sp. Ha25
7.5
-
 pullulytic activity Halorubrum sp. Ha25

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8.5 pH 5.5: about 45% of maximal activity, pH 8.5: about 45% of maximal activity Halorubrum sp. Ha25