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Literature summary for 3.2.1.41 extracted from

  • Singh, R.; Saini, G.; Kennedy, J.
    Maltotriose syrup preparation from pullulan using pullulanase (2010), Carbohydr. Polym., 80, 402-408.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
Brij-35 stimulates at 0.025-0.10% v/v Bacillus acidopullulyticus
SDS stimulates at 0.025-0.10% v/v Bacillus acidopullulyticus
Triton X-100 stimulates at 0.025-0.10% v/v Bacillus acidopullulyticus
Tween 80 stimulates at 0.025-0.10% v/v Bacillus acidopullulyticus

Application

Application Comment Organism
food industry maltotriose syrup preparation from pullulan using pullulanase, overview. In a batch system, pullulanase hydrolyzes 94.25% of purified pullulan and the resultant syrup contains 3.8 mg/ml of maltotriose Bacillus acidopullulyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics and thermodynamics, overview Bacillus acidopullulyticus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates 2.1fold Bacillus acidopullulyticus
Mn2+ activates 1.8fold Bacillus acidopullulyticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bacillus acidopullulyticus pullulanase is a debranching enzyme which hydrolyses the alpha-1,6-glucosidic linkages in pullulan and other amylaceous polysaccharides ?
-
?

Organism

Organism UniProt Comment Textmining
Bacillus acidopullulyticus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Bacillus acidopullulyticus
-

Storage Stability

Storage Stability Organism
purified enzyme, 6 h, 50°C, pH 5.0, completely stable Bacillus acidopullulyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dextran + H2O
-
Bacillus acidopullulyticus ?
-
?
additional information pullulanase is a debranching enzyme which hydrolyses the alpha-1,6-glucosidic linkages in pullulan and other amylaceous polysaccharides Bacillus acidopullulyticus ?
-
?
additional information pullulanase effectively hydrolyzes pullulan, soluble starch and dextran Bacillus acidopullulyticus ?
-
?
pullulan + H2O substrate from Aureobasidium pullulans strain FB-1 Bacillus acidopullulyticus ?
-
?
soluble starch + H2O
-
Bacillus acidopullulyticus ?
-
?

Synonyms

Synonyms Comment Organism
More the enzyme belongs to a family of 13 glycosyl hydrolases, also called the alpha-amylase family Bacillus acidopullulyticus
Pullulan 6-glucanohydrolase
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Bacillus acidopullulyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Bacillus acidopullulyticus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 70
-
Bacillus acidopullulyticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
thermal inactivation kinetics, overview Bacillus acidopullulyticus
50 70 irreversible inactivation of free pullulanase, 92.51% and 82.52% of maximal activity at 55°C and 60°C, respectively, rapid decrease at 70°C Bacillus acidopullulyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Bacillus acidopullulyticus

pH Range

pH Minimum pH Maximum Comment Organism
4.5 6.5 over 89% of maximal activity between pH 5.0 and pH 6.5, over 75% at pH 4.5 Bacillus acidopullulyticus