Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-cyclodextrin | - |
Oryza sativa | |
Co2+ | 5 mM, at least 60% inhibition | Oryza sativa | |
Cu2+ | 5 mM, at least 60% inhibition | Oryza sativa | |
Hg2+ | 5 mM, at least 60% inhibition | Oryza sativa | |
pullulan | substrate inhibition above 0.1% | Oryza sativa | |
Zn2+ | 5 mM, at least 60% inhibition | Oryza sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
1 * 100000, SDS-PAGE | Oryza sativa |
105000 | - |
gel filtration | Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Oryza sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | pullulanase from germinating and nongerminating seed are the same protein | Oryza sativa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | - |
Oryza sativa | maltooligosaccharides + maltose | 34.1% of the rate with pullulan | ? | |
beta-limit dextrin + H2O | - |
Oryza sativa | ? | 19.5% of the rate with pullulan | ? | |
glycogen + H2O | - |
Oryza sativa | maltooligosaccharides + maltose | 6.3% of the rate with pullulan | ? | |
additional information | enzyme exhibits alpha-glucosyltransfer activity and produces an alpha-(1-6) linked compound of two maltotriose molecules from pullulan | Oryza sativa | ? | - |
? | |
pullulan + H2O | preferred substrate | Oryza sativa | maltotriose + ? | - |
? | |
soluble starch | - |
Oryza sativa | maltotriose + maltose | 15.8% of the rate with pullulan | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 100000, SDS-PAGE | Oryza sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Oryza sativa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
stable up to | Oryza sativa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.18 | - |
amylopectin | 37°C, pH 6.0 | Oryza sativa | |
2.89 | - |
soluble starch | 37°C, pH 6.0 | Oryza sativa | |
3.31 | - |
beta-limit dextrin | 37°C, pH 6.0 | Oryza sativa | |
23.1 | - |
pullulan | 37°C, pH 6.0 | Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Oryza sativa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8 | at 30°C | Oryza sativa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Oryza sativa | - |
- |
4.7 |