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Literature summary for 3.2.1.41 extracted from

  • Duffner, F.; Bertoldo, C.; Andersen, J.T.; Wagner, K.; Antranikian, G.
    A new thermoactive pullulanase from Desulfurococcus mucosus: Cloning, sequencing, purification, and characterization of the recombinant enzyme after expression in Bacillus subtilis (2000), J. Bacteriol., 182, 6331-6338.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.41

Cloned(Commentary)

Cloned (Comment) Organism
-
 Desulfurococcus mucosus

Inhibitors

Inhibitors Comment Organism Structure
ammonium sulfate 0.5 mM, 36% residual activity Desulfurococcus mucosus
CuCl2 5 mM, no residual activity Desulfurococcus mucosus
EDTA 5 mM, 63% residual activity Desulfurococcus mucosus
additional information not inhibitory: iodoacetamide, Triton X-100, Mn2+, Mg2+, Ca2+ Desulfurococcus mucosus
N-bromosuccinimide 0.01%, no residual activity Desulfurococcus mucosus
sodium dodecylsulfate 1 mM, no residual activity Desulfurococcus mucosus
Urea 4 M, 81% residual activity Desulfurococcus mucosus
ZnCl2 5 mM, 51% residual activity Desulfurococcus mucosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74000
-
 x * 74000, deduced from gene sequence, mature protein Desulfurococcus mucosus

Organism

Organism UniProt Comment Textmining
Desulfurococcus mucosus Q9HHB0
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification enzyme contains signal sequence Desulfurococcus mucosus

Purification (Commentary)

Purification (Comment) Organism
expressed in Bacillus subtilis Desulfurococcus mucosus

Source Tissue

Source Tissue Comment Organism Textmining
culture fluid
-
 Desulfurococcus mucosus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
26
-
 pH 5.5, 80°C Desulfurococcus mucosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O 35% of activity compared to pullulan Desulfurococcus mucosus ?
-
 ?
amylose + H2O 77% of activity compared to pullulan Desulfurococcus mucosus ?
-
 ?
beta-cyclodextrin + H2O 21% of activity compared to pullulan Desulfurococcus mucosus ?
-
 ?
gamma-cyclodextrin + H2O 71% of activity compared to pullulan Desulfurococcus mucosus ?
-
 ?
pullulan + H2O
-
 Desulfurococcus mucosus ?
-
 ?
starch + H2O 62% of activity compared to pullulan Desulfurococcus mucosus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 74000, deduced from gene sequence, mature protein Desulfurococcus mucosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
-
 Desulfurococcus mucosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
 1 h, stable Desulfurococcus mucosus
80
-
 1 h, 90% residual activity Desulfurococcus mucosus
85
-
 1 h, 45% residual activity Desulfurococcus mucosus
90
-
 1 h, no residual activity Desulfurococcus mucosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Desulfurococcus mucosus