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Literature summary for 3.2.1.41 extracted from
- Purification and properties of a thermostable pullulanase from Clostridium thermohydrosulfurogenes EM1 which hydrolyses both alpha-1,6 and alpha-1,4-glycosidic linkages (1990), Appl. Microbiol. Biotechnol., 33, 511-518.
No PubMed abstract available
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Thermoanaerobacter thermohydrosulfuricus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | - |
SDS-PAGE | Thermoanaerobacter thermohydrosulfuricus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | Thermoanaerobacter thermohydrosulfuricus | branched polysaccharides | maltooligosaccharides + maltose | - |
? |  |
| pullulan + H2O | Thermoanaerobacter thermohydrosulfuricus | - |
maltotriose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoanaerobacter thermohydrosulfuricus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | maltooligosaccharides + maltose | - |
? | |
| amylopectin + H2O | branched polysaccharides | Thermoanaerobacter thermohydrosulfuricus | maltooligosaccharides + maltose | - |
? | |
| amylose + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | maltooligosaccharides + maltose | - |
? | |
| glycogen + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | maltooligosaccharides + maltose | - |
? | |
| maltoheptaose + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | maltotriose + maltose + D-glucose | - |
? | |
| maltohexaose + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | maltotriose + maltose + D-glucose + maltotetraose | - |
? | |
| maltopentaose + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | maltotriose + maltose | - |
? | |
| maltotetraose + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | maltose + glucose + maltotriose | - |
? | |
| pullulan + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | maltotriose + ? | - |
? | |
| soluble starch | - |
Thermoanaerobacter thermohydrosulfuricus | maltotriose + maltose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Thermoanaerobacter thermohydrosulfuricus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pullulanase type II | hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides | Thermoanaerobacter thermohydrosulfuricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 65 | - |
Thermoanaerobacter thermohydrosulfuricus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 75 | - |
Thermoanaerobacter thermohydrosulfuricus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6 | - |
Thermoanaerobacter thermohydrosulfuricus |
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