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Literature summary for 3.2.1.41 extracted from

  • Melasniemi, H.
    Characterization of alpha-amylase and pullulanase activities of Clostridium thermohydrosulfuricum. Evidence for a novel thermostable amylase (1987), Biochem. J., 246, 193-197.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Thermoanaerobacter thermohydrosulfuricus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Thermoanaerobacter thermohydrosulfuricus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Thermoanaerobacter thermohydrosulfuricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
amylopectin + H2O Thermoanaerobacter thermohydrosulfuricus branched polysaccharides maltooligosaccharides + maltose
-
?
amylopectin + H2O Thermoanaerobacter thermohydrosulfuricus E 101-69 branched polysaccharides maltooligosaccharides + maltose
-
?
pullulan + H2O Thermoanaerobacter thermohydrosulfuricus
-
maltotriose + ?
-
?
pullulan + H2O Thermoanaerobacter thermohydrosulfuricus E 101-69
-
maltotriose + ?
-
?

Organism

Organism UniProt Comment Textmining
Thermoanaerobacter thermohydrosulfuricus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O
-
Thermoanaerobacter thermohydrosulfuricus maltooligosaccharides + maltose
-
?
amylopectin + H2O branched polysaccharides Thermoanaerobacter thermohydrosulfuricus maltooligosaccharides + maltose
-
?
amylopectin + H2O
-
Thermoanaerobacter thermohydrosulfuricus E 101-69 maltooligosaccharides + maltose
-
?
amylopectin + H2O branched polysaccharides Thermoanaerobacter thermohydrosulfuricus E 101-69 maltooligosaccharides + maltose
-
?
amylose + H2O
-
Thermoanaerobacter thermohydrosulfuricus maltooligosaccharides + maltose
-
?
amylose + H2O
-
Thermoanaerobacter thermohydrosulfuricus E 101-69 maltooligosaccharides + maltose
-
?
pullulan + H2O
-
Thermoanaerobacter thermohydrosulfuricus maltotriose + ?
-
?
pullulan + H2O
-
Thermoanaerobacter thermohydrosulfuricus E 101-69 maltotriose + ?
-
?

Synonyms

Synonyms Comment Organism
pullulanase type II hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides Thermoanaerobacter thermohydrosulfuricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
Thermoanaerobacter thermohydrosulfuricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
not stable above Thermoanaerobacter thermohydrosulfuricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
-
Thermoanaerobacter thermohydrosulfuricus

pH Range

pH Minimum pH Maximum Comment Organism
4 7.5
-
Thermoanaerobacter thermohydrosulfuricus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 10.5
-
Thermoanaerobacter thermohydrosulfuricus