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Literature summary for 3.2.1.41 extracted from
- Purification and characterization of thermostable pullulanase from Bacillus stearothermophilus and molecular cloning and expression of the gene in Bacillus subtilis (1988), Appl. Environ. Microbiol., 54, 2881-2883.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Bacillus subtilis | Geobacillus stearothermophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Geobacillus stearothermophilus |  |
| EDTA | - |
Geobacillus stearothermophilus | |
| Zn2+ | - |
Geobacillus stearothermophilus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Geobacillus stearothermophilus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | highly stimulated | Geobacillus stearothermophilus | |
| Ca2+ | highly stimulated | Geobacillus stearothermophilus | |
| Co2+ | slightly stimulated | Geobacillus stearothermophilus | |
| Fe2+ | slightly stimulated | Geobacillus stearothermophilus | |
| Mg2+ | slightly stimulated | Geobacillus stearothermophilus | |
| Mn2+ | slightly stimulated | Geobacillus stearothermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 83000 | - |
gel electrophoresis | Geobacillus stearothermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | Geobacillus stearothermophilus | branched polysaccharides | maltooligosaccharides + maltose | - |
? | |
| pullulan + H2O | Geobacillus stearothermophilus | - |
maltotriose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| of the recombinant protein | Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 49.6 | - |
- |
Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Geobacillus stearothermophilus | maltooligosaccharides + maltose | - |
? | |
| amylopectin + H2O | branched polysaccharides | Geobacillus stearothermophilus | maltooligosaccharides + maltose | - |
? | |
| pullulan + H2O | - |
Geobacillus stearothermophilus | maltotriose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 83000, gel electrophoresis | Geobacillus stearothermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pullulanase type II | hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides | Geobacillus stearothermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Geobacillus stearothermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Geobacillus stearothermophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | - |
Geobacillus stearothermophilus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | - |
Geobacillus stearothermophilus |
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