Application | Comment | Organism |
---|---|---|
drug development | the characteristics (good thermostability, wide range of pH-stability with the optimum pH of 5.0 and temperature of 60°C, not greatly affected by representative metal ions, excellent tolerance abilities against glucose and ethanol) of the enzyme suggest that it should be considered a potential new biocatalyst for food and drug industrial applications | Aspergillus niger |
food industry | the characteristics (good thermostability, wide range of pH-stability with the optimum pH of 5.0 and temperature of 60°C, not greatly affected by representative metal ions, excellent tolerance abilities against glucose and ethanol) of the enzyme suggest that it should be considered a potential new biocatalyst for food and drug industrial applications | Aspergillus niger |
Cloned (Comment) | Organism |
---|---|
overexpression in Pichia pastoris GS115s | Aspergillus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 100 mM, complete inactivation | Aspergillus niger | |
Fe3+ | 10 mM, 46% inhibition. 100 mM, 91% inhibition | Aspergillus niger |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM, 1.1fold activation. 100 mM, 1.4fold activation | Aspergillus niger | |
Co2+ | 10 mM, 1.1fold activation. 100 mM, 1.6fold activation | Aspergillus niger | |
Cu2+ | 10 mM, 1.2fold activation. 100 mM, 1.3fold activation | Aspergillus niger | |
Fe2+ | 10 mM, 1.1fold activation | Aspergillus niger | |
Mg2+ | 10 mM or 100 mM, 1.1fold activation | Aspergillus niger | |
Mn2+ | 100 mM, 1.5fold activation | Aspergillus niger | |
Ni2+ | 100 mM, 1.5fold activation | Aspergillus niger | |
Zn2+ | 10 mM, 1.1fold activation. 100 mM, 1.5fold activation | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | R9W5L0 | - |
- |
Aspergillus niger JMU-TS528 | R9W5L0 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
711.9 | - |
activity of the recombinant overexpressed enzyme, pH and temperature not specified in the publication | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-L-rhamnopyranoside + H2O | 70.1% of the activity compared to naringin | Aspergillus niger | 4-nitrophenol + alpha-L-rhamnopyranose | - |
? | |
4-nitrophenyl alpha-L-rhamnopyranoside + H2O | 70.1% of the activity compared to naringin | Aspergillus niger JMU-TS528 | 4-nitrophenol + alpha-L-rhamnopyranose | - |
? | |
hesperidin + H2O | 144.9% of the activity compared to naringin | Aspergillus niger | hesperetin 7-O-beta-D-glucoside + alpha-L-rhamnose | - |
? | |
hesperidin + H2O | 144.9% of the activity compared to naringin | Aspergillus niger JMU-TS528 | hesperetin 7-O-beta-D-glucoside + alpha-L-rhamnose | - |
? | |
additional information | the enzyme posseses broad substrate specificities by hydrolyzing alpha-1,2, alpha-1,3 alpha-1,4, and alpha-1,6 linkages to beta-D-glucosides | Aspergillus niger | ? | - |
? | |
additional information | the enzyme posseses broad substrate specificities by hydrolyzing alpha-1,2, alpha-1,3 alpha-1,4, and alpha-1,6 linkages to beta-D-glucosides | Aspergillus niger JMU-TS528 | ? | - |
? | |
myricetrin + H2O | 22.5% of the activity compared to naringin | Aspergillus niger | 3,5,7-trihydroxy-2-(3,4,5-trihydroxyphenyl)-4H-chromen-4-one + alpha-L-rhamnose | - |
? | |
naringin + H2O | - |
Aspergillus niger | beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose | - |
? | |
naringin + H2O | - |
Aspergillus niger JMU-TS528 | beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose | - |
? | |
rutin + H2O | 136.7% of the activity compared to naringin | Aspergillus niger | isoquercitrin + alpha-L-rhamnose | - |
? | |
rutin + H2O | 136.7% of the activity compared to naringin | Aspergillus niger JMU-TS528 | isoquercitrin + alpha-L-rhamnose | - |
? | |
saikosaponin C + H2O | 9.19% of the activity compared to naringin | Aspergillus niger | (3beta,16beta)-16-hydroxy-13,28-epoxyolean-11-en-3-yl) 6-O-beta-D-glucopyranosyl-beta-D-glucopyranoside + alpha-L-rhamnose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Aspergillus niger |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | 30°C: about 65% of maximal activity, 70°C: about 55% of maximal activity | Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
half-life time: 32.66 h | Aspergillus niger |
55 | - |
half-life time: 15.44 h | Aspergillus niger |
60 | - |
half-life time: 2.59 h | Aspergillus niger |
65 | - |
half-life time: 18.7 min | Aspergillus niger |
70 | - |
half-life time: 4.6 min | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Aspergillus niger |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 11 | 24 h, 4°C, more than 45% of its enzymatic activity is retained | Aspergillus niger |