Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-rhamnose | compeitive | Acrostalagmus luteoalbus | |
additional information | neither divalent cations such as Ca2+, Mg2+, Mn2+, and Co2+ nor reducing agents such as 2-mercaptoethanol and dithiothreitol show effects on enzyme activtiy | Acrostalagmus luteoalbus | |
Zn2+ | 0.2 mM, complete inhibition | Acrostalagmus luteoalbus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.38 | - |
4-nitrophenyl-alpha-L-rhamnoside | pH 8.0, 55°C | Acrostalagmus luteoalbus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
109000 | - |
x * 109000, SDS-PAGE | Acrostalagmus luteoalbus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acrostalagmus luteoalbus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
from cells grown on rhamnose as sole carbon source | Acrostalagmus luteoalbus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
526 | - |
pH 9.5, 37°C | Acrostalagmus luteoalbus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-L-rhamnoside + H2O | - |
Acrostalagmus luteoalbus | alpha-L-rhamnose + 4-nitrophenol | - |
? | |
hesperidin + H2O | - |
Acrostalagmus luteoalbus | hesperetin 7-O-beta-D-glucoside + L-rhamnose | - |
? | |
additional information | enzyme is able to hydrolyze alpha-1,2- and alpha-1,6-linkages to beta-D-glucosides in addition to direct linkages from the L-rhamnose residue to the aglycon | Acrostalagmus luteoalbus | ? | - |
? | |
naringin + H2O | - |
Acrostalagmus luteoalbus | 4',5,7-trihydroxyflavanone 7-O-beta-D-glucoside + L-rhamnose | - |
? | |
quercitrin + H2O | - |
Acrostalagmus luteoalbus | quercetin + L-rhamnose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 109000, SDS-PAGE | Acrostalagmus luteoalbus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Acrostalagmus luteoalbus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable up to 4 h | Acrostalagmus luteoalbus |
55 | - |
4 h, 81% residual activity | Acrostalagmus luteoalbus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Acrostalagmus luteoalbus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | 24 h, without substrate, 95% residual activity | Acrostalagmus luteoalbus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
4.7 | - |
L-rhamnose | pH 9.5, 37°C | Acrostalagmus luteoalbus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Acrostalagmus luteoalbus | isoelectric focusing | - |
4.6 |