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Literature summary for 3.2.1.40 extracted from

  • Rojas, N.L.; Voget, C.E.; Hours, R.A.; Cavalitto, S.F.
    Purification and characterization of a novel alkaline alpha-L-rhamnosidase produced by Acrostalagmus luteo albus (2011), J. Ind. Microbiol. Biotechnol., 38, 1515-1522.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
L-rhamnose compeitive Acrostalagmus luteoalbus
additional information neither divalent cations such as Ca2+, Mg2+, Mn2+, and Co2+ nor reducing agents such as 2-mercaptoethanol and dithiothreitol show effects on enzyme activtiy Acrostalagmus luteoalbus
Zn2+ 0.2 mM, complete inhibition Acrostalagmus luteoalbus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.38
-
4-nitrophenyl-alpha-L-rhamnoside pH 8.0, 55°C Acrostalagmus luteoalbus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
109000
-
x * 109000, SDS-PAGE Acrostalagmus luteoalbus

Organism

Organism UniProt Comment Textmining
Acrostalagmus luteoalbus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from cells grown on rhamnose as sole carbon source Acrostalagmus luteoalbus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
526
-
pH 9.5, 37°C Acrostalagmus luteoalbus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-rhamnoside + H2O
-
Acrostalagmus luteoalbus alpha-L-rhamnose + 4-nitrophenol
-
?
hesperidin + H2O
-
Acrostalagmus luteoalbus hesperetin 7-O-beta-D-glucoside + L-rhamnose
-
?
additional information enzyme is able to hydrolyze alpha-1,2- and alpha-1,6-linkages to beta-D-glucosides in addition to direct linkages from the L-rhamnose residue to the aglycon Acrostalagmus luteoalbus ?
-
?
naringin + H2O
-
Acrostalagmus luteoalbus 4',5,7-trihydroxyflavanone 7-O-beta-D-glucoside + L-rhamnose
-
?
quercitrin + H2O
-
Acrostalagmus luteoalbus quercetin + L-rhamnose
-
?

Subunits

Subunits Comment Organism
? x * 109000, SDS-PAGE Acrostalagmus luteoalbus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Acrostalagmus luteoalbus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
stable up to 4 h Acrostalagmus luteoalbus
55
-
4 h, 81% residual activity Acrostalagmus luteoalbus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Acrostalagmus luteoalbus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5 9 24 h, without substrate, 95% residual activity Acrostalagmus luteoalbus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.7
-
L-rhamnose pH 9.5, 37°C Acrostalagmus luteoalbus

pI Value

Organism Comment pI Value Maximum pI Value
Acrostalagmus luteoalbus isoelectric focusing
-
4.6