Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | a slight activating influence on isoform RhaB1 is observed for EDTA at 10 mM | Lactiplantibacillus plantarum |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 S1 cells | Lactiplantibacillus plantarum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | less effective inhibitor, when the 1,10-phenanthroline concentration is raised to 10 mM, the activity of isoform RhaB1 decreases by 50%; less effective inhibitor, when the 1,10-phenanthroline concentration is raised to 10 mM, the activity of isoform RhaB2 decreases by 50% | Lactiplantibacillus plantarum | |
Cu2+ | - |
Lactiplantibacillus plantarum | |
Fe2+ | - |
Lactiplantibacillus plantarum | |
L-rhamnose | isoform RhaB1 is slightly inhibited by L-rhamnose, showing noncompetitive inhibition | Lactiplantibacillus plantarum | |
Mn2+ | - |
Lactiplantibacillus plantarum | |
additional information | dithiothreitol and beta-mercaptoethanol have no or little effect on activity; dithiothreitol and beta-mercaptoethanol have no or little effect on activity | Lactiplantibacillus plantarum | |
phosphoramidon | isoform RhaB1 shows 23.8% residual activity at 1 mM; isoform RhaB2 shows 42.5% residual activity at 1 mM | Lactiplantibacillus plantarum | |
polyhexamethylene biguanide | 1 mM reduces the activity of isoform RhaB1 to 10.9% and isoform RhaB2 to 36.0% residual activity; 1 mM reduces the activity of isoform RhaB1 to 10.9% residual activity | Lactiplantibacillus plantarum | |
SDS | SDS produces partial inactivation; SDS produces partial inactivation | Lactiplantibacillus plantarum | |
thiorphan | less effective inhibitor; less effective inhibitor | Lactiplantibacillus plantarum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.51 | - |
4-nitrophenyl alpha-L-rhamnopyranoside | isoform RhaB2 | Lactiplantibacillus plantarum | |
0.57 | - |
4-nitrophenyl alpha-L-rhamnopyranoside | isoform RhaB1 | Lactiplantibacillus plantarum | |
0.78 | - |
hesperidin | isoform RhaB2 | Lactiplantibacillus plantarum | |
1.06 | - |
rutin | isoform RhaB2 | Lactiplantibacillus plantarum | |
1.77 | - |
rutin | isoform RhaB1 | Lactiplantibacillus plantarum | |
3.39 | - |
hesperidin | isoform RhaB1 | Lactiplantibacillus plantarum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Lactiplantibacillus plantarum | |
Co2+ | - |
Lactiplantibacillus plantarum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactiplantibacillus plantarum | C4PG45 | isoform RhaB2 | - |
Lactiplantibacillus plantarum | C4PG47 | isoform RhaB1 | - |
Lactiplantibacillus plantarum NCC245 | C4PG45 | isoform RhaB2 | - |
Lactiplantibacillus plantarum NCC245 | C4PG47 | isoform RhaB1 | - |
Purification (Comment) | Organism |
---|---|
HiTrap chelating column chromatography, gel filtration | Lactiplantibacillus plantarum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-L-rhamnopyranoside + H2O | - |
Lactiplantibacillus plantarum | 4-nitrophenol + alpha-L-rhamnopyranose | - |
? | |
4-nitrophenyl alpha-L-rhamnopyranoside + H2O | - |
Lactiplantibacillus plantarum NCC245 | 4-nitrophenol + alpha-L-rhamnopyranose | - |
? | |
4-nitrophenyl alpha-L-rhamnoside + H2O | - |
Lactiplantibacillus plantarum | alpha-L-rhamnose + 4-nitrophenol | - |
? | |
4-nitrophenyl alpha-L-rhamnoside + H2O | - |
Lactiplantibacillus plantarum NCC245 | alpha-L-rhamnose + 4-nitrophenol | - |
? | |
hesperidin + H2O | 11.2% relative activity compared to rutin | Lactiplantibacillus plantarum | hesperetin 7-O-glucoside + alpha-L-rhamnose | - |
? | |
hesperidin + H2O | isoform RhaB2 exhibits highest activity against hesperidin | Lactiplantibacillus plantarum | hesperetin 7-O-glucoside + alpha-L-rhamnose | - |
? | |
hesperidin + H2O | isoform RhaB2 exhibits highest activity against hesperidin | Lactiplantibacillus plantarum NCC245 | hesperetin 7-O-glucoside + alpha-L-rhamnose | - |
? | |
hesperidin + H2O | 11.2% relative activity compared to rutin | Lactiplantibacillus plantarum NCC245 | hesperetin 7-O-glucoside + alpha-L-rhamnose | - |
? | |
additional information | no activity is detected towards the alpha-1,2 linkage in naringin | Lactiplantibacillus plantarum | ? | - |
? | |
additional information | no activity towards quercitin, gellan gum, vitexin 2-O-rhamnoside, and alpha-chaconine | Lactiplantibacillus plantarum | ? | - |
? | |
additional information | no activity is detected towards the alpha-1,2 linkage in naringin | Lactiplantibacillus plantarum NCC245 | ? | - |
? | |
additional information | no activity towards quercitin, gellan gum, vitexin 2-O-rhamnoside, and alpha-chaconine | Lactiplantibacillus plantarum NCC245 | ? | - |
? | |
proscillaridin A + H2O | 0.4% relative activity compared to hesperidin | Lactiplantibacillus plantarum | ? | - |
? | |
proscillaridin A + H2O | 4.5% relative activity compared to rutin | Lactiplantibacillus plantarum | ? | - |
? | |
rutin + H2O | 10.1% relative activity compared to hesperidin | Lactiplantibacillus plantarum | isoquercitrin + alpha-L-rhamnose | - |
? | |
rutin + H2O | isoform RhaB1 exhibits highest activity against rutin | Lactiplantibacillus plantarum | isoquercitrin + alpha-L-rhamnose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RhaB1 | isoform | Lactiplantibacillus plantarum |
RhaB2 | isoform | Lactiplantibacillus plantarum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
isoform RhaB1 | Lactiplantibacillus plantarum |
60 | - |
isoform RhaB2 | Lactiplantibacillus plantarum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | the enzyme shows relative activities over 64% from 30 to 60°C | Lactiplantibacillus plantarum |
50 | 70 | isoform RhaB2 is most active at temperatures between 50 and 70°C, still showing 70% relative activity at 70°C | Lactiplantibacillus plantarum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 65 | isoform RhaB2 retains 43% and 25% activity after 5 h incubation at 55°C and 65°C, respectively | Lactiplantibacillus plantarum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
isoform RhaB2 | Lactiplantibacillus plantarum |
7 | - |
isoform RhaB1 | Lactiplantibacillus plantarum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.4 | 5.5 | isoform RhaB2 maintains over 60% of its activity in the pH range from 4.4 to 5.5 | Lactiplantibacillus plantarum |
5 | 7.5 | isoform RhaB1 maintains over 60% of its activity in the pH range from 5.0 to 7.5 | Lactiplantibacillus plantarum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | isoform RhaB1 remains active over a broad range of pH values, showing 90-100% remaining relative activities from pH 4.0 to 7.5, and over 40% at pH 3 and 9, after 5 h at 37°C | Lactiplantibacillus plantarum |
3 | 9 | the stability of isoform RhaB2 is lower than that of isoform RhaB1, with 80-85% enzymatic activity from pH 5.0 to 7.0, and only 17% and 12% at pH 3.0 and 9.0, respectively | Lactiplantibacillus plantarum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
19.88 | - |
L-rhamnose | isoform RhaB1 | Lactiplantibacillus plantarum |
Organism | Comment | Expression |
---|---|---|
Lactiplantibacillus plantarum | the enzyme activity is downregulated by D-glucose | down |
Lactiplantibacillus plantarum | the enzyme activity is upregulated by L-rhamnose | up |