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Literature summary for 3.2.1.40 extracted from

  • Koseki, T.; Mese, Y.; Nishibori, N.; Masaki, K.; Fujii, T.; Handa, T.; Yamane, Y.; Shiono, Y.; Murayama, T.; Iefuji, H.
    Characterization of an alpha-L-rhamnosidase from Aspergillus kawachii and its gene (2008), Appl. Microbiol. Biotechnol., 80, 1007-1013.
    View publication on PubMed
Search for more literature for 3.2.1.40

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
 x * 90000, SDS-PAGE, x * 68000, calculated Aspergillus luchuensis
90000
-
 x * 90000, SDS-PAGE, x * 68000, calculated Aspergillus luchuensis

Organism

Organism UniProt Comment Textmining
Aspergillus luchuensis A9ZT55
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein degree of N-glycosylation about 22%, sequence contains 13 putative N-glycosylation sites Aspergillus luchuensis

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus luchuensis

Source Tissue

Source Tissue Comment Organism Textmining
culture fluid
-
 Aspergillus luchuensis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17.9
-
 substrate 4-nitrophenyl alpha-L-rhamnopyranoside, pH 4.0, 40°C Aspergillus luchuensis
29.5
-
 substrate hesperidin, pH 4.0, 40°C Aspergillus luchuensis
85.3
-
 substrate naringin, pH 4.0, 40°C Aspergillus luchuensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
-
 Aspergillus luchuensis 4-nitrophenol + alpha-L-rhamnose
-
 ?
hesperidin + H2O substrate naringin is preferred over hesperidin Aspergillus luchuensis hesperetin 7-glucoside + alpha-L-rhamnose
-
 ?
additional information enzyme is able to hydrolyze alpha-(1-2) amd alpha-(1-6) glycosidic bonds Aspergillus luchuensis ?
-
 ?
naringin + H2O substrate naringin is preferred over hesperidin Aspergillus luchuensis 4',5,7-trihydroxyflavanone-7-beta-D-glucoside + L-rhamnose
-
 ?

Subunits

Subunits Comment Organism
? x * 90000, SDS-PAGE, x * 68000, calculated Aspergillus luchuensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Aspergillus luchuensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 1 h, 80% residual activity Aspergillus luchuensis
72
-
 T50 value Aspergillus luchuensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Aspergillus luchuensis

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus luchuensis calculated
-
 5.1