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Literature summary for 3.2.1.40 extracted from
- The thermostable alpha-L-rhamnosidase RamA of Clostridium stercorarium: biochemical characterization and primary structure of a bacterial alpha-L-rhamnoside hydrolase, a new type of inverting glycoside hydrolase (2000), Mol. Microbiol., 35, 173-179.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli, gene has no homology to other glycoside hydrolases | Thermoclostridium stercorarium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha-L-rhamnose | - |
Thermoclostridium stercorarium |  |
| Cu2+ | - |
Thermoclostridium stercorarium | |
| Hg2+ | - |
Thermoclostridium stercorarium | |
| p-hydroxymercuribenzoate | - |
Thermoclostridium stercorarium | |
| SDS | - |
Thermoclostridium stercorarium | |
| Zn2+ | - |
Thermoclostridium stercorarium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 95000 | - |
SDS-PAGE, amino acid analysis | Thermoclostridium stercorarium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-L-rhamnoside + H2O | Thermoclostridium stercorarium | alpha-L-rhamnose in glycolipids or glycosides | alpha-L-rhamnose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoclostridium stercorarium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| of the recombinant protein | Thermoclostridium stercorarium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.46 | - |
hesperidin | Thermoclostridium stercorarium |
| 1.5 | - |
naringin | Thermoclostridium stercorarium |
| 82 | - |
p-nitrophenyl-alpha-L-rhamnopyranoside | Thermoclostridium stercorarium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4',5,7-trihydroxyflavanone 7-rhamnoglucoside + H2O | - |
Thermoclostridium stercorarium | 4',5,7-trihydroxyflavanone 7-glucoside + alpha-L-rhamnose | - |
? | |
| alpha-L-rhamnoside + H2O | alpha-L-rhamnose in glycolipids or glycosides | Thermoclostridium stercorarium | alpha-L-rhamnose + ? | - |
? | |
| hesperidin + H2O | - |
Thermoclostridium stercorarium | hesperetin 7-glucoside + alpha-L-rhamnose | - |
? | |
| naringin + H2O | - |
Thermoclostridium stercorarium | beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose | - |
? | |
| p-nitrophenyl alpha-L-rhamnoside + H2O | - |
Thermoclostridium stercorarium | p-nitrophenol + alpha-L-rhamnose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 95000, SDS-PAGE, amino acid analysis | Thermoclostridium stercorarium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-L-rhamnosidase Ram A | - |
Thermoclostridium stercorarium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Thermoclostridium stercorarium |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | 68 | - |
Thermoclostridium stercorarium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Thermoclostridium stercorarium |
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