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Literature summary for 3.2.1.40 extracted from
- Purification and characterization of an alpha-L-rhamnosidase from Aspergillus niger (1997), FEMS Microbiol. Lett., 157, 279-283.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha-L-rhamnose | competitive | Aspergillus niger |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.9 | - |
p-nitrophenyl-alpha-L-rhamnopyranoside | - |
Aspergillus niger | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 85000 | - |
gel filtration, SDS-PAGE | Aspergillus niger |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-L-rhamnoside + H2O | Aspergillus niger | alpha-L-rhamnose in glycolipids or glycosides | alpha-L-rhamnose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 12% N-glycosylated | Aspergillus niger |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus niger |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Aspergillus niger | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-phenylethyl beta-D-rutinoside + H2O | - |
Aspergillus niger | 2-phenylethyl beta-D-glucopyranoside + alpha-L-rhamnose | - |
? | |
| 4',5,7-trihydroxyflavanone 7-rhamnoglucoside + H2O | - |
Aspergillus niger | 4',5,7-trihydroxyflavanone 7-glucoside + alpha-L-rhamnose | - |
? | |
| alpha-L-rhamnoside + H2O | alpha-L-rhamnose in glycolipids or glycosides | Aspergillus niger | alpha-L-rhamnose + ? | - |
? | |
| geranyl-beta-D-rutinoside + H2O | - |
Aspergillus niger | geranyl-beta-D-glycoside + alpha-L-rhamnose | - |
? | |
| hesperidin + H2O | - |
Aspergillus niger | hesperetin 7-glucoside + alpha-L-rhamnose | - |
? | |
| naringin + H2O | - |
Aspergillus niger | beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose | - |
? | |
| p-nitrophenyl alpha-L-rhamnoside + H2O | - |
Aspergillus niger | p-nitrophenol + alpha-L-rhamnose | - |
? | |
| p-nitrophenyl-alpha-L-rhamnopyranoside + H2O | - |
Aspergillus niger | p-nitrophenol + alpha-L-rhamnopyranose | - |
? | |
| rutin + H2O | - |
Aspergillus niger | 3-glycosylquercetin + alpha-L-rhamnose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Aspergillus niger |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
no loss of activity after 4 h | Aspergillus niger |
| 50 | - |
15% loss of activity after 4 h | Aspergillus niger |
| 60 | - |
40% loss of activity after 1 h | Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 5 | - |
Aspergillus niger |
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