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Literature summary for 3.2.1.4 extracted from

  • Zhao, J.; Shi, P.; Li, Z.; Yang, P.; Luo, H., Bai, Y., Wang, Y.; Yao, B.
    Two neutral thermostable cellulases from Phialophora sp. G5 act synergistically in the hydrolysis of filter paper (2012), Bioresour. Technol., 121, 404-410.
    View publication on PubMed
Search for more literature for 3.2.1.4

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 118.4% relative activity Phialophora sp. CGMCC 3328
2-mercaptoethanol 129.7% relative activity Phialophora sp. CGMCC 3328
EDTA 99.4% relative activity Phialophora sp. CGMCC 3328
EDTA 99.7% relative activity Phialophora sp. CGMCC 3328

Application

Application Comment Organism
industry textile and detergent industry, combination of CBH6A and EgGH45 Phialophora sp. CGMCC 3328

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Phialophora sp. CGMCC 3328

Inhibitors

Inhibitors Comment Organism Structure
Pb2+ no remaining activity; no remaining activity Phialophora sp. CGMCC 3328
SDS 10 mM, 35% remaining activity; 10 mM, 54% remaining activity Phialophora sp. CGMCC 3328

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 carboxymethyl cellulose 46.2 mg/l and vmax: 17.1 micromol/min/mg, pH 7, 60°C Phialophora sp. CGMCC 3328
additional information
-
 carboxymethyl cellulose 71.9 mg/l and vmax: 28.6 micromol/min/mg, pH 7, 65°C Phialophora sp. CGMCC 3328

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 57.8% relative activity Phialophora sp. CGMCC 3328
Ca2+ 85.5% relative activity Phialophora sp. CGMCC 3328
Co2+ 69.2% relative activity Phialophora sp. CGMCC 3328
Co2+ 85.5% relative activity Phialophora sp. CGMCC 3328
Cr3+ 76.7% relative activity Phialophora sp. CGMCC 3328
Cr3+ 84.1% relative activity Phialophora sp. CGMCC 3328
Cu2+ 116.1% relative activity Phialophora sp. CGMCC 3328
Cu2+ 118% relative activity Phialophora sp. CGMCC 3328
Fe3+ 114.3% relative activity Phialophora sp. CGMCC 3328
Fe3+ 87.5% relative activity Phialophora sp. CGMCC 3328
K+ 86.2% relative activity Phialophora sp. CGMCC 3328
K+ 96.1% relative activity Phialophora sp. CGMCC 3328
Li+ 84.6% relative activity Phialophora sp. CGMCC 3328
Li+ 97% relative activity Phialophora sp. CGMCC 3328
Mg2+ 108.5% relative activity Phialophora sp. CGMCC 3328
Mg2+ 99.8% relative activity Phialophora sp. CGMCC 3328
Na+ 97.6% relative activity Phialophora sp. CGMCC 3328
Na+ 99.4% relative activity Phialophora sp. CGMCC 3328
Ni2+ 100.8% relative activity Phialophora sp. CGMCC 3328
Ni2+ 107.1% relative activity Phialophora sp. CGMCC 3328
Zn2+ 110% relative activity Phialophora sp. CGMCC 3328
Zn2+ 99.9% relative activity Phialophora sp. CGMCC 3328

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 difference to 56000 due to posttranslational modification during heterologous expression in Pichia pastoris, SDS-PAGE Phialophora sp. CGMCC 3328
36000
-
 difference to 56000 duet to posttranslational modification during heterologous expression in Pichia pastoris, SDS-PAGE Phialophora sp. CGMCC 3328
56000
-
 difference to 36000 due to posttranslational modification during heterologous expression in Pichia pastoris, SDS-PAGE Phialophora sp. CGMCC 3328
56000
-
 difference to 36000 duet to posttranslational modification during heterologous expression in Pichia pastoris, SDS-PAGE Phialophora sp. CGMCC 3328

Organism

Organism UniProt Comment Textmining
Phialophora sp. CGMCC 3328 F2VRY9 HM003039; G5 CGMCC 3328
-
Phialophora sp. CGMCC 3328 F2VRZ0 HM003040; G5 CGMCC 3328
-

Purification (Commentary)

Purification (Comment) Organism
centrifuged at 12,000 x g for 10 min to remove cell depris, hot shock, ultrafiltration, dialysis, gel filtration Phialophora sp. CGMCC 3328

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
avicel + H2O 14% of the activity with carboxymethyl-cellulose Phialophora sp. CGMCC 3328 ?
-
 ?
avicel + H2O 17.7% of the activity with carboxymethyl-cellulose Phialophora sp. CGMCC 3328 ?
-
 ?
barley glucan + H2O 120% of the activity with carboxymethyl-cellulose Phialophora sp. CGMCC 3328 ?
-
 ?
barley glucan + H2O 143% of the activity with carboxymethyl-cellulose Phialophora sp. CGMCC 3328 ?
-
 ?
carboxymethyl cellulose + H2O
-
 Phialophora sp. CGMCC 3328 ?
-
 ?
filter paper + H2O 4% of the activity with carboxymethyl-cellulose. CBH6A and EgGH45, synergic effect, showing the highest efficiency in the ratio at 80:20 Phialophora sp. CGMCC 3328 ?
-
 ?
filter paper + H2O 8% of the activity with carboxymethyl-cellulose. CBH6A and EgGH45, synergic effect, showing the highest efficiency in the ratio at 80:20 Phialophora sp. CGMCC 3328 ?
-
 ?
additional information no activity against birchwood xylan, 4-nitrophenyl cellobiose, 4-nitrophenyl beta-D-glucoside Phialophora sp. CGMCC 3328 ?
-
 ?

Synonyms

Synonyms Comment Organism
cbh6A gene name, consists of a family 1 CBM and a catalytic domain of glycosyl hydrolase family 6 cellobiohydrolases Phialophora sp. CGMCC 3328
egGH45 gene name, contains a catalytic domain of family 45 endoglucanases Phialophora sp. CGMCC 3328

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 50-70, determnined at 30-80°C, pH 6 for 30 min Phialophora sp. CGMCC 3328
65
-
 50-70, determnined at 30-80°C, pH 7 for 30 min Phialophora sp. CGMCC 3328

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 30°C: 20% relative activity, respectively, 70°C: 70% relative activity Phialophora sp. CGMCC 3328
30 70 30°C: 30% relative activity, respectively, 70°C: 70% relative activity Phialophora sp. CGMCC 3328

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 CBH6A and EgGH45, 90% activity for 10 min Phialophora sp. CGMCC 3328
80
-
 CBH6A and EgGH45, 80% activity for 10 min Phialophora sp. CGMCC 3328
90
-
 CBH6A and EgGH45, 47% activity for 10 min Phialophora sp. CGMCC 3328

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8 EgGH45, measured in various buffers at pH 3-11 (McIlvaine, Tris-HCl, glycine-NaOH) at 60°C for 30 min Phialophora sp. CGMCC 3328
7
-
 CBH6A, measured in various buffers at pH 3-11 (McIlvaine, Tris-HCl, glycine-NaOH) at 60°C for 30 min Phialophora sp. CGMCC 3328

pH Range

pH Minimum pH Maximum Comment Organism
3 11 pH 3: 20% relative activity, pH 11: 35% relatice activity at 65°C Phialophora sp. CGMCC 3328
3 11 pH 3: 25% relative activity, pH 11: 40% relatice activity at 65°C Phialophora sp. CGMCC 3328

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 11 80-100% acticity, enzymes are preincubated in the buffers without substrate at 37°C for 2 h, and the residual activities are measured at optimal conditions for 30 min, 60°C and pH 6.0 for EgGH45 Phialophora sp. CGMCC 3328
2 11 80-100% activity, enzymes are preincubated in the buffers without substrate at 37°C for 2 h, and the residual activities are measured at optimal conditions for 30 min, 65°C and pH 7 Phialophora sp. CGMCC 3328