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Literature summary for 3.2.1.4 extracted from

  • Dojnov, B.; Gruji?, M.; Vuj?i?, Z.
    Reliable simultaneous zymographic method of characterization of cellulolytic enzymes from fungal cellulase complex (2015), Electrophoresis, 36, 1724-1727.
    View publication on PubMed

Application

Application Comment Organism
analysis method for zymographic detection of specific cellulases in a complex (endocellulase, exocellulase, and cellobiase) from crude fermentation extracts, after a single electrophoretic separation. Cellulases are printed onto a membrane and, subsequently, substrate gel. Cellobiase isoforms are detected on the membrane using esculine as substrate, endocellulase isoforms on substrate gel with copolymerized carboxymethyl cellulose, while exocellulase isoforms are detected in electrophoresis gel with 4-methylumbelliferyl-beta-D-cellobioside Trichoderma sp.

Organism

Organism UniProt Comment Textmining
Trichoderma sp.
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