Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.4 extracted from

  • Chen, Z.; Pereira, J.H.; Liu, H.; Tran, H.M.; Hsu, N.S.; Dibble, D.; Singh, S.; Adams, P.D.; Sapra, R.; Hadi, M.Z.; Simmons, B.A.; Sale, K.L.
    Improved activity of a thermophilic cellulase, Cel5A, from Thermotoga maritima on ionic liquid pretreated switchgrass (2013), PLoS ONE, 8, e79725.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
A153V mutant with increased activity on 1-ethyl-3-methylimidazolium acetate pretreated biomass. Mutant displays 130% of wild-type activity with carboxymethyl cellulose Thermotoga maritima
H138R mutant with increased activity on 1-ethyl-3-methylimidazolium acetate pretreated biomass. Mutant displays 130% of wild-type activity with carboxymethyl cellulose Thermotoga maritima
additional information engineering of mutants of isoform Cel5A with higher activity on 1-ethyl-3-methylimidazolium acetate pretreated biomass by screening of a random mutagenesis library. Twelve mutants with 25–42% improvement in specific activity on carboxymethyl cellulose and up to 30% improvement on ionic-liquid pretreated switchgrass could be isolated and characterized. Mmost of the mutations in the improved variants are located distally to the active site on the protein surface and are not directly involved with substrate binding Thermotoga maritima
N236D mutant with increased activity on 1-ethyl-3-methylimidazolium acetate pretreated biomass. Mutant displays 142% of wild-type activity with carboxymethyl cellulose Thermotoga maritima
Y66F mutant with increased activity on 1-ethyl-3-methylimidazolium acetate pretreated biomass. Mutant displays 132% of wild-type activity with carboxymethyl cellulose Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima Q9X273
-
-
Thermotoga maritima DSM 3109 Q9X273
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethyl cellulose + H2O
-
Thermotoga maritima ?
-
?
carboxymethyl cellulose + H2O
-
Thermotoga maritima DSM 3109 ?
-
?

Synonyms

Synonyms Comment Organism
Cel5A
-
Thermotoga maritima