Protein Variants | Comment | Organism |
---|---|---|
A153V | mutant with increased activity on 1-ethyl-3-methylimidazolium acetate pretreated biomass. Mutant displays 130% of wild-type activity with carboxymethyl cellulose | Thermotoga maritima |
H138R | mutant with increased activity on 1-ethyl-3-methylimidazolium acetate pretreated biomass. Mutant displays 130% of wild-type activity with carboxymethyl cellulose | Thermotoga maritima |
additional information | engineering of mutants of isoform Cel5A with higher activity on 1-ethyl-3-methylimidazolium acetate pretreated biomass by screening of a random mutagenesis library. Twelve mutants with 2542% improvement in specific activity on carboxymethyl cellulose and up to 30% improvement on ionic-liquid pretreated switchgrass could be isolated and characterized. Mmost of the mutations in the improved variants are located distally to the active site on the protein surface and are not directly involved with substrate binding | Thermotoga maritima |
N236D | mutant with increased activity on 1-ethyl-3-methylimidazolium acetate pretreated biomass. Mutant displays 142% of wild-type activity with carboxymethyl cellulose | Thermotoga maritima |
Y66F | mutant with increased activity on 1-ethyl-3-methylimidazolium acetate pretreated biomass. Mutant displays 132% of wild-type activity with carboxymethyl cellulose | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | Q9X273 | - |
- |
Thermotoga maritima DSM 3109 | Q9X273 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxymethyl cellulose + H2O | - |
Thermotoga maritima | ? | - |
? | |
carboxymethyl cellulose + H2O | - |
Thermotoga maritima DSM 3109 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cel5A | - |
Thermotoga maritima |