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Literature summary for 3.2.1.4 extracted from
- Heterologous expression of hyperthermophilic cellulases of archaea Pyrococcus sp. by fungus Talaromyces cellulolyticus (2015), J. Ind. Microbiol. Biotechnol., 42, 137-141.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| heterologous expression in Talaromyces cellulolyticus under the control of a glucoamylase promoter of Talaromyces cellulolyticus. The characteristics of the enzyme are almost same as those prepared by Escherichia coli | Pyrococcus horikoshii |
| heterologous expression in Talaromyces cellulolyticus under the control of a glucoamylase promoter of Talaromyces cellulolyticus. The characteristics of the enzyme are almost same as those prepared by Escherichia coli | Pyrococcus furiosus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
x * 28000, SDS-PAGE | Pyrococcus furiosus |
| 40000 | - |
x * 40000, SDS-PAGE | Pyrococcus horikoshii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | E7FHY8 | - |
- |
| Pyrococcus horikoshii | O58925 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pyrococcus horikoshii |
- |
Pyrococcus furiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethyl cellulose + H2O | - |
Pyrococcus horikoshii | ? | - |
? |  |
| carboxymethyl cellulose + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 28000, SDS-PAGE | Pyrococcus furiosus |
| ? | x * 40000, SDS-PAGE | Pyrococcus horikoshii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EfPh | - |
Pyrococcus horikoshii |
| EGPf | - |
Pyrococcus furiosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
assay at | Pyrococcus horikoshii |
| 80 | - |
assay at | Pyrococcus furiosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
assay at | Pyrococcus horikoshii |
| 5 | - |
assay at | Pyrococcus furiosus |
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Release 2023.1 (January 2023)
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