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Literature summary for 3.2.1.4 extracted from
- Bacillus pumilus S124A carboxymethyl cellulase; a thermo stable enzyme with a wide substrate spectrum utility (2014), Int. J. Biol. Macromol., 67, 132-139.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 1 mM, 40% residual activity | Bacillus pumilus |  |
| EDTA | 27% residual activity | Bacillus pumilus | |
| Fe2+ | 1 mM, 37% residual activity | Bacillus pumilus | |
| Hg2+ | 1 mM, 30% residual activity | Bacillus pumilus | |
| Phenylmethyl sulfonylfluoride | 1 mM, 30% residual activity | Bacillus pumilus | |
| sodium dodecylsulfate | 1%, 32% residual activity | Bacillus pumilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
carboxymethyl cellulose | Km value is 2.1 mg/ml, pH 6.0, 50°C | Bacillus pumilus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus pumilus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
x * 40000, SDS-PAGE | Bacillus pumilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus pumilus | - |
- |
- |
| Bacillus pumilus S124A | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus pumilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethyl cellulose + H2O | - |
Bacillus pumilus | ? | - |
? | |
| carboxymethyl cellulose + H2O | - |
Bacillus pumilus S124A | ? | - |
? | |
| filter paper + H2O | - |
Bacillus pumilus | ? | - |
? | |
| filter paper + H2O | - |
Bacillus pumilus S124A | ? | - |
? | |
| additional information | enzyme displays both beta-glucosidase, EC 3.2.1.21, and cellulase activities | Bacillus pumilus | ? | - |
? | |
| additional information | enzyme displays both beta-glucosidase, EC 3.2.1.21, and cellulase activities | Bacillus pumilus S124A | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, SDS-PAGE | Bacillus pumilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Bacillus pumilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Bacillus pumilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | more than 75% of maximum activity | Bacillus pumilus |
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Release 2023.1 (January 2023)
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