Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.4 extracted from

  • Cheng, Y.; Ko, T.; Huang, J.; Wu, T.; Lin, C.; Luo, W.; Li, Q.; Ma, Y.; Huang, C.; Wang, A.; Liu, J.; Guo, R.
    Enhanced activity of Thermotoga maritima cellulase 12A by mutating a unique surface loop (2012), Appl. Microbiol. Biotechnol., 95, 661-669.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
mutants Y16G, Y61GG and Y61del in complex with substrate beta-glucan. Mutations at the Tyr61 site do not affect the overall protein structure, but local perturbations might diminish the substrate-binding strength Thermotoga maritima

Protein Variants

Protein Variants Comment Organism
R60A about 45% of wild-type activity Thermotoga maritima
R60K about 70% of wild-type activity Thermotoga maritima
Y61A about 120% of wild-type activity Thermotoga maritima
Y61del about 10% of wild-type activity Thermotoga maritima
Y61F about 140% of wild-type activity Thermotoga maritima
Y61G about 170% of wild-type activity. Mutant also shows a wider range of working temperatures than does the wild type, along with retention of the hyperthermostability. The kcat and Km values of Y61G are both higher than those of the wild type. The higher endoglucanase activity is probably due to facile dissociation of the cleaved sugar moiety at the reducing end Thermotoga maritima
Y61GG insertion mutant, about 40% of wild-type activity Thermotoga maritima
Y61R about 70% of wild-type activity Thermotoga maritima
Y61W about 80% of wild-type activity Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
beta-Glucan Km value for wild-type is 2.08 mg/ml, for mutant Y61G 3.75 mg/ml, pH 5.0, 85°C Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima Q60032
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-glucan + H2O
-
Thermotoga maritima ?
-
?

Synonyms

Synonyms Comment Organism
CelA
-
Thermotoga maritima
cellulase 12A
-
Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
wild-type Thermotoga maritima
100
-
mutant Y61G Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
half-life 8 h, both wild-type and mutant Y61G Thermotoga maritima
95
-
half-life 40 min, both wild-type and mutant Y61G Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
791
-
beta-Glucan wild-type, pH 5.0, 85°C Thermotoga maritima
1155
-
beta-Glucan mutant Y61G, pH 5.0, 85°C Thermotoga maritima

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
beta-Glucan Kcat/Km value for wild-type is 402 mg per s and ml, for mutant Y61G 313 mg per s and ml, pH 5.0, 85°C Thermotoga maritima