Application | Comment | Organism |
---|---|---|
biofuel production | the enzyme is a tool for biomass conversion. The recombinant enzyme acts in high concentrations of ionic liquids and can therefore degrade alpha-cellulose or even complex cell wall preparations under those pretreatment conditions. The enzymatic conversion of lignocellulosic plant biomass into fermentable sugars is a crucial step in the production of biofuels | Saccharolobus solfataricus |
Cloned (Comment) | Organism |
---|---|
expression in tobacco leaf, including ER-tag | Saccharolobus solfataricus |
expression of SSO1354 in Nicotiana tabacum | Saccharolobus solfataricus |
General Stability | Organism |
---|---|
the enzyme tolerates high salt concentrations, with 49.5% residual activity in the presence of 2.4 M NaCl, and 46.5% residual activity in the presence of 2.4 M CaCl2 | Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 46.5% residual activity in the presence of 2.4 M CaCl2 | Saccharolobus solfataricus | |
NaCl | 49.5% residual activity in the presence of 2.4 M NaCl | Saccharolobus solfataricus |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | 15% (v/v), 49% loss of activity, nearly complete inhibition at 30% (v/v) | Saccharolobus solfataricus |
Acetone | 15% v/v, 51% residual activity | Saccharolobus solfataricus |
dimethyl sulfoxide | 52% (v/v), 21% loss of activity | Saccharolobus solfataricus |
DMSO | 15% v/v, 75% residual activity | Saccharolobus solfataricus |
Ethanol | 15% v/v, 48% residual activity | Saccharolobus solfataricus |
Ethanol | 15% (v/v), 25% loss of activity | Saccharolobus solfataricus |
Methanol | 15% (v/v), 21% loss of activity, nearly complete inhibition at 30% (v/v) | Saccharolobus solfataricus |
Methanol | 15% v/v, 79% residual activity | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q97YG7 | - |
- |
Saccharolobus solfataricus P2 | Q97YG7 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
recombinant protein | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl beta-D-cellobioside + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
barley beta-D-glucan + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
barley beta-glucan + H2O | 136% of the activity with carboxymethyl cellulose | Saccharolobus solfataricus | ? | - |
? | |
carboxymethyl cellulose + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
carboxymethyl cellulose + H2O | - |
Saccharolobus solfataricus P2 | ? | - |
? | |
lichenan + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
lichenan + H2O | 130% of the activity with carboxymethyl cellulose | Saccharolobus solfataricus | ? | - |
? | |
lichenan + H2O | - |
Saccharolobus solfataricus P2 | ? | - |
? | |
lichenan + H2O | 130% of the activity with carboxymethyl cellulose | Saccharolobus solfataricus P2 | ? | - |
? | |
additional information | the enzyme has a beta-1,4 hydrolytic mode of action. It shows no significant activity on PCM3/pachyman, microcrystalline cellulose, or on oat xylan. Concentrated solutions (80–90% v/v) of ionic liquids such as 1-ethyl-3-methylimidazolium acetate and 1,3-dimethylimidazolium dimethylphosphate are able to dissolve crystalline cellulose like AvicelW and cell wall polysaccharides, making them more accessible to hydrolytic enzymes | Saccharolobus solfataricus | ? | - |
? | |
additional information | no substrates: PCM3/pachyman, avicel, oat xylan | Saccharolobus solfataricus | ? | - |
? | |
additional information | the enzyme has a beta-1,4 hydrolytic mode of action. It shows no significant activity on PCM3/pachyman, microcrystalline cellulose, or on oat xylan. Concentrated solutions (80–90% v/v) of ionic liquids such as 1-ethyl-3-methylimidazolium acetate and 1,3-dimethylimidazolium dimethylphosphate are able to dissolve crystalline cellulose like AvicelW and cell wall polysaccharides, making them more accessible to hydrolytic enzymes | Saccharolobus solfataricus P2 | ? | - |
? | |
additional information | no substrates: PCM3/pachyman, avicel, oat xylan | Saccharolobus solfataricus P2 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SSO1354 | - |
Saccharolobus solfataricus |
SSO1354 | locus name | Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
24% residual activity | Saccharolobus solfataricus |
75 | 100 | 75°C: 60% of maximal activity, 100°C: about 80% of maximal activity | Saccharolobus solfataricus |
99 | - |
79% residual activity | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
pH 4.5, 100 min, 50% loss of activity | Saccharolobus solfataricus |
90 | - |
pH 4.5, 100 min, 50% residual activity | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Saccharolobus solfataricus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.7 | 6.5 | pH 2.7: 58% of maximal activity, pH 6.5: 62% of maximal activity. Minimal activity below pH 2.2 and above pH 7.0 | Saccharolobus solfataricus |
2.7 | - |
58% residual activity | Saccharolobus solfataricus |
6.5 | - |
62% residual activity | Saccharolobus solfataricus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
90°C, 100 min, 50% loss of activity | Saccharolobus solfataricus |
4.5 | - |
90°C, 100 min, 50% residual activity | Saccharolobus solfataricus |
General Information | Comment | Organism |
---|---|---|
physiological function | recombinant expression in tobacco leaf. Activity ranges from 92 to 488 nmol 4-methylumbelliferyl-beta-D-cellobioside/min/mg, the morphology and growth behavior of the transgenic plants are not different to those of wild-type plants | Saccharolobus solfataricus |