Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Pyrococcus furiosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cellobiose | - |
Pyrococcus furiosus | |
D-glucono-1,5-lactone | - |
Pyrococcus furiosus | |
D-glucose | - |
Pyrococcus furiosus | |
laminaribiose | - |
Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.061 | - |
4-nitrophenyl cellotrioside | pH 6.0, 95°C | Pyrococcus furiosus | |
0.12 | - |
4-nitrophenyl cellotetraoside | pH 6.0, 95°C | Pyrococcus furiosus | |
0.15 | - |
4-nitrophenyl cellobioside | pH 6.0, 95°C | Pyrococcus furiosus | |
0.36 | - |
4-nitrophenyl cellopentaoside | pH 6.0, 95°C | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35900 | - |
x * 35900, calculated from sequence | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q9V2T0 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucoside + H2O | low activity | Pyrococcus furiosus | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl cellobioside + H2O | - |
Pyrococcus furiosus | 4-nitrophenol + cellobiose | - |
? | |
4-nitrophenyl cellopentaoside + H2O | - |
Pyrococcus furiosus | 4-nitrophenol + cellopentaose | - |
? | |
4-nitrophenyl cellotetraoside + H2O | - |
Pyrococcus furiosus | 4-nitrophenol + cellotetraose | - |
? | |
4-nitrophenyl cellotrioside + H2O | - |
Pyrococcus furiosus | 4-nitrophenol + cellotriose | - |
? | |
barley beta-glucan + H2O | the highest specific activity toward polysaccharides occurs with mixed-linkage (1->3),(1->4)-beta-D-glucans such as barley beta-glucan and lichenan | Pyrococcus furiosus | ? | - |
? | |
cellohexaose + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
cellopentaose + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
lichenan + H2O | the highest specific activity toward polysaccharides occurs with mixed-linkage (1->3),(1->4)-beta-D-glucans such as barley beta-D-glucan and lichenan | Pyrococcus furiosus | ? | - |
? | |
additional information | EglA hydrolyzes shorter cellodextrins (DP <5) as well as the amorphous portions of polysaccharides which contain only beta-1,4 bonds such as carboxymethyl cellulose, microcrystalline cellulose, Whatman paper, and cotton linter. Kinetics studies with cellooliogsaccharides and p-nitrophenyl-cellooligosaccharides indicated that the enzyme had three glucose binding subsites (-I, -II, and -III) for the nonreducing end and two glucose binding subsites (+I and +II) for the reducing end from the scissile glycosidic linkage. No activity is detected on solely beta-1,3-linked oligosaccharides or polysaccharides | Pyrococcus furiosus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35900, calculated from sequence | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
EglA | - |
Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
- |
Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
half-life: 40 h | Pyrococcus furiosus |
112 | - |
denaturing temperature | Pyrococcus furiosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.79 | - |
4-nitrophenyl cellobioside | pH 6.0, 95°C | Pyrococcus furiosus | |
1.4 | - |
4-nitrophenyl cellopentaoside | pH 6.0, 95°C | Pyrococcus furiosus | |
3.6 | - |
4-nitrophenyl cellotetraoside | pH 6.0, 95°C | Pyrococcus furiosus | |
8.3 | - |
4-nitrophenyl cellotrioside | pH 6.0, 95°C | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Pyrococcus furiosus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
D-glucono-1,5-lactone | pH 6.0, 95°C | Pyrococcus furiosus | |
1.6 | - |
laminaribiose | pH 6.0, 95°C | Pyrococcus furiosus | |
3 | - |
cellobiose | pH 6.0, 95°C | Pyrococcus furiosus | |
90 | - |
D-glucose | pH 6.0, 95°C | Pyrococcus furiosus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
4-nitrophenyl beta-D-glucoside | pH 6.0, 95°C | Pyrococcus furiosus | |
6.5 | - |
4-nitrophenyl cellopentaoside | pH 6.0, 95°C | Pyrococcus furiosus | |
8.7 | - |
4-nitrophenyl cellobioside | pH 6.0, 95°C | Pyrococcus furiosus | |
49 | - |
4-nitrophenyl cellotetraoside | pH 6.0, 95°C | Pyrococcus furiosus | |
220 | - |
4-nitrophenyl cellotrioside | pH 6.0, 95°C | Pyrococcus furiosus |