Cloned (Comment) | Organism |
---|---|
expression of His-tagged CcCel6C in Escherichia coli strain BL21(DE3) | Coprinopsis cinerea |
Crystallization (Comment) | Organism |
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purified recombinant His-tagged CcCel6C unbound and in complex with 4-nitrophenyl beta-D-cellotrioside and cellobiose, hanging drop vapour diffusion method, mixing of 0.001 ml of 21.5 mg/ml containing protein solution with 0.001 ml of well solution containing 100 mM HEPES/KOH, pH 7.0, 30% PEG 8000, 150 mM magnesium acetate, soaking of crystals in 60 mM 4-nitrophenyl beta-D-cellotrioside for 2 h or 220 mM cellobiose for 5 min in well solution for complex formation, X-ray diffraction structure determination and analysis at 1.6 A, 1.4 A, and 1.2 A resolution, respectively | Coprinopsis cinerea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Coprinopsis cinerea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged CcCel6C from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Coprinopsis cinerea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxymethyl cellulose + H2O | - |
Coprinopsis cinerea | ? | - |
? | |
additional information | CcCel6C exhibits high cellobiohydrolase activity | Coprinopsis cinerea | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | CcCel6C consists of a distorted seven-stranded beta/alpha barrel and has an enclosed tunnel, that remains wide, structure, overview | Coprinopsis cinerea |
Synonyms | Comment | Organism |
---|---|---|
CcCel6C | - |
Coprinopsis cinerea |
cellobiohydrolase | - |
Coprinopsis cinerea |
More | the enzyme belongs to the glycoside hydrolase family 6 | Coprinopsis cinerea |