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Literature summary for 3.2.1.4 extracted from

  • Liu, Y.; Yoshida, M.; Kurakata, Y.; Miyazaki, T.; Igarashi, K.; Samejima, M.; Fukuda, K.; Nishikawa, A.; Tonozuka, T.
    Crystal structure of a glycoside hydrolase family 6 enzyme, CcCel6C, a cellulase constitutively produced by Coprinopsis cinerea (2010), FEBS J., 277, 1532-1542.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged CcCel6C in Escherichia coli strain BL21(DE3) Coprinopsis cinerea

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant His-tagged CcCel6C unbound and in complex with 4-nitrophenyl beta-D-cellotrioside and cellobiose, hanging drop vapour diffusion method, mixing of 0.001 ml of 21.5 mg/ml containing protein solution with 0.001 ml of well solution containing 100 mM HEPES/KOH, pH 7.0, 30% PEG 8000, 150 mM magnesium acetate, soaking of crystals in 60 mM 4-nitrophenyl beta-D-cellotrioside for 2 h or 220 mM cellobiose for 5 min in well solution for complex formation, X-ray diffraction structure determination and analysis at 1.6 A, 1.4 A, and 1.2 A resolution, respectively Coprinopsis cinerea

Organism

Organism UniProt Comment Textmining
Coprinopsis cinerea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged CcCel6C from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Coprinopsis cinerea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethyl cellulose + H2O
-
Coprinopsis cinerea ?
-
?
additional information CcCel6C exhibits high cellobiohydrolase activity Coprinopsis cinerea ?
-
?

Subunits

Subunits Comment Organism
More CcCel6C consists of a distorted seven-stranded beta/alpha barrel and has an enclosed tunnel, that remains wide, structure, overview Coprinopsis cinerea

Synonyms

Synonyms Comment Organism
CcCel6C
-
Coprinopsis cinerea
cellobiohydrolase
-
Coprinopsis cinerea
More the enzyme belongs to the glycoside hydrolase family 6 Coprinopsis cinerea