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Literature summary for 3.2.1.4 extracted from
- Crystallization and preliminary X-ray analysis of endoglucanase from Pyrococcus horikoshii (2008), Acta Crystallogr. Sect. F, 64, 1169-1171.
Application
| Application | Comment | Organism |
|---|---|---|
| industry | potent enzyme for industrial bioprocesses, including the bio-polishing of cotton products, food processing and bioethanol production | Pyrococcus horikoshii |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| X-ray diffraction analysis of crystals of the wild-type full-length EGPh is unsuccessful, sitting-drop vapour-diffusion method | Pyrococcus horikoshii |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | EGPhDELTAC5, truncated form of the enzyme shows similar enzymatic properties to the wild-type protein. EGPhDELTAC10, lacking ten residues at the C-terminus showed significantly decreased activity. Three truncated mutants (EGPhDELTAN5, EGPhDELTAC5 and EGPhDELTAN5C5) which show no change in enzymatic properties are prepared and screened for crystallization | Pyrococcus horikoshii |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
EGPhDELTAC5, SDS-PAGE | Pyrococcus horikoshii |
| 43000 | - |
wild-type protein, SDS-PAGE | Pyrococcus horikoshii |
| 43970 | - |
theoretically, EGPhDELTAC5, truncated form of the enzyme | Pyrococcus horikoshii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellulose + H2O | Pyrococcus horikoshii | - |
cellooligosaccharide | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus horikoshii | O58925 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellulose + H2O | - |
Pyrococcus horikoshii | cellooligosaccharide | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-1,4-glucanase | - |
Pyrococcus horikoshii |
| Cellulase | - |
Pyrococcus horikoshii |
| EGPh | - |
Pyrococcus horikoshii |
| endoglucanase | - |
Pyrococcus horikoshii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
hyperthermostable | Pyrococcus horikoshii |
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Release 2023.1 (January 2023)
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