Cloned (Comment) | Organism |
---|---|
selenomethionine-substituted CMCax is overexpressed in Escherichia coli B834 Codon Plus (DE3)-IRL (Stratagene) with a (His)6-tag at the N-terminus in place of the signal peptide | Komagataeibacter xylinus |
Crystallization (Comment) | Organism |
---|---|
a single crystal of CMCax (Crystal I) is grown at 20°C by the hanging drop vapor diffusion method against a reservoir solution containing 100 mM sodium citrate buffer, pH 4.4, 15% (w/v) PEG 4,000, 3% (w/v) PEG 8,000, and 20 mM ammonium sulfate. Crystal belongs to the space group P6(1) with unit cell dimensions of a = b = 89.1 A and c = 94.2 A. Another crystal of larger size (Crystal II) is obtained under the above conditions but with the addition of 10 mM cellobiose. Prior to X-ray diffraction experiments, the crystal is rapidly soaked in reservoir solution supplemented with 20% (v/v) glycerol, and flash-cooled under a stream of nitrogen gas. The crystal diffracted to a resolution of 1.65 A | Komagataeibacter xylinus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Komagataeibacter xylinus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Komagataeibacter xylinus |
Synonyms | Comment | Organism |
---|---|---|
endo-beta-1,4-glucanase CMCax | - |
Komagataeibacter xylinus |