Literature summary for 3.2.1.4 extracted from

Kim, H.W.; Takagi, Y.; Hagihara, Y.; Ishikawa, K.
Analysis of the putative substrate binding region of hyperthermophilic endoglucanase from Pyrococcus horikoshii (2007), Biosci. Biotechnol. Biochem., 71, 2585-2587.

Search for more literature for 3.2.1.4

Cloned(Commentary)

Cloned (Comment)	Organism
expression of mutant enzymes in Escherichia coli	Pyrococcus horikoshii

Protein Variants

Protein Variants	Comment	Organism
C159A	kcat/KM for p-nitrophenyl cellobiose is 1.8fold lower than wild-type value	Pyrococcus horikoshii
E163A	kcat/KM for p-nitrophenyl cellobiose is 6fold lower than wild-type value	Pyrococcus horikoshii
G158A	kcat/KM for p-nitrophenyl cellobiose is 2fold lower than wild-type value	Pyrococcus horikoshii
H155A	kcat/KM for p-nitrophenyl cellobiose is 140fold lower than wild-type value	Pyrococcus horikoshii
H161A	kcat/KM for p-nitrophenyl cellobiose is neatrly identical to wild-type value	Pyrococcus horikoshii
I157A	kcat/KM for p-nitrophenyl cellobiose is nearly identical to wild-type value	Pyrococcus horikoshii
I162A	kcat/KM for p-nitrophenyl cellobiose is 140fold lower than wild-type value	Pyrococcus horikoshii
P164A	kcat/KM for p-nitrophenyl cellobiose is 6fold lower than wild-type value	Pyrococcus horikoshii
R156A	kcat/KM for p-nitrophenyl cellobiose is 10fold lower than wild-type value	Pyrococcus horikoshii
T160A	kcat/KM for p-nitrophenyl cellobiose is nearly identical to wild-type value	Pyrococcus horikoshii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.88	-	p-nitrophenyl cellobiose	pH 5.5, 50°C, mutant enzyme I157A	Pyrococcus horikoshii
0.92	-	p-nitrophenyl cellobiose	pH 5.5, 50°C, mutant enzyme T160A	Pyrococcus horikoshii
1.12	-	p-nitrophenyl cellobiose	pH 5.5, 50°C, wild-type enzyme	Pyrococcus horikoshii
1.23	-	p-nitrophenyl cellobiose	pH 5.5, 50°C, mutant enzyme H161A	Pyrococcus horikoshii
1.37	-	p-nitrophenyl cellobiose	pH 5.5, 50°C, mutant enzyme G158A	Pyrococcus horikoshii
1.55	-	p-nitrophenyl cellobiose	pH 5.5, 50°C, mutant enzyme E163A	Pyrococcus horikoshii
1.58	-	p-nitrophenyl cellobiose	pH 5.5, 50°C, mutant enzyme P164A	Pyrococcus horikoshii
1.9	-	p-nitrophenyl cellobiose	pH 5.5, 50°C, mutant enzyme C159A	Pyrococcus horikoshii
4.57	-	p-nitrophenyl cellobiose	pH 5.5, 50°C, mutant enzyme I162A	Pyrococcus horikoshii
5.85	-	p-nitrophenyl cellobiose	pH 5.5, 50°C, mutant enzyme R156A	Pyrococcus horikoshii
6.69	-	p-nitrophenyl cellobiose	pH 5.5, 50°C, mutant enzyme H155A	Pyrococcus horikoshii

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus horikoshii	O58925	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
avicel + H2O	-	Pyrococcus horikoshii	?	-	?
carboxymethyl cellulose + H2O	-	Pyrococcus horikoshii	?	-	?
crystalline cellulase + H2O	-	Pyrococcus horikoshii	?	-	?
p-nitrophenyl cellobiose + H2O	-	Pyrococcus horikoshii	p-nitrophenol + cellobiose	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.003	-	p-nitrophenyl cellobiose	pH 5.5, 50°C, mutant enzyme I162A	Pyrococcus horikoshii
0.006	-	p-nitrophenyl cellobiose	pH 5.5, 50°C, mutant enzyme H155A	Pyrococcus horikoshii
0.036	-	p-nitrophenyl cellobiose	pH 5.5, 50°C, mutant enzyme E163A	Pyrococcus horikoshii
0.037	-	p-nitrophenyl cellobiose	pH 5.5, 50°C, mutant enzyme P164A	Pyrococcus horikoshii
0.083	-	p-nitrophenyl cellobiose	pH 5.5, 50°C, mutant enzyme R156A	Pyrococcus horikoshii
0.092	-	p-nitrophenyl cellobiose	pH 5.5, 50°C, mutant enzyme G158A	Pyrococcus horikoshii
0.115	-	p-nitrophenyl cellobiose	pH 5.5, 50°C, mutant enzyme I157A	Pyrococcus horikoshii
0.129	-	p-nitrophenyl cellobiose	pH 5.5, 50°C, mutant enzyme T160A	Pyrococcus horikoshii
0.144	-	p-nitrophenyl cellobiose	pH 5.5, 50°C, mutant enzyme C159A	Pyrococcus horikoshii
0.157	-	p-nitrophenyl cellobiose	pH 5.5, 50°C, wild-type enzyme	Pyrococcus horikoshii
0.167	-	p-nitrophenyl cellobiose	pH 5.5, 50°C, mutant enzyme H161A	Pyrococcus horikoshii

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Release 2023.1 (January 2023)