Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | uncultured microorganism |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CuCl2 | 82% inhibition | uncultured microorganism | |
EDTA | 53% inhibition | uncultured microorganism | |
SDS | 66% inhibition | uncultured microorganism | |
ZnCl2 | 47% inhibition | uncultured microorganism |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km-value for carboxymethyl cellulose is 16.07 mg/ml | uncultured microorganism |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | stimulation to 122% of control | uncultured microorganism | |
CoCl2 | stimulation to 137% of control | uncultured microorganism | |
CrCl3 | stimulation to 119% of control | uncultured microorganism |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
uncultured microorganism | - |
in rabbit caecum | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | uncultured microorganism |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxymethyl cellulose + H2O | - |
uncultured microorganism | ? | - |
? | |
cellopentaose + H2O | - |
uncultured microorganism | cellobiose + cellotriose | - |
? | |
cellotetraose + H2O | - |
uncultured microorganism | 2 cellobiose | - |
? | |
cellotriose + H2O | slightly degraded | uncultured microorganism | D-glucose + cellobiose | - |
? | |
additional information | no hydrolysis of cellobiose | uncultured microorganism | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
umcel5G | - |
uncultured microorganism |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
uncultured microorganism |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
30 min, less than 10% loss of activity | uncultured microorganism |
55 | - |
unstable above | uncultured microorganism |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | substrate: carboxymethyl cellulose | uncultured microorganism |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 10 | weak activity at pH 10.0 and below pH 3.5, substrate: carboxymethyl cellulose | uncultured microorganism |
4.5 | 8 | pH 4.5: about 60% of maximal activity, pH 8.0: about 40% of maximal activity | uncultured microorganism |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | enzyme retains more than 90% activity after storage for 24 h at broad pH ranging from pH 5.0 to 9.0 | uncultured microorganism |