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Literature summary for 3.2.1.4 extracted from

  • Arai, T.; Kosugi, A.; Chan, H.; Koukiekolo, R.; Yukawa, H.; Inui, M.; Doi, R.H.
    Properties of cellulosomal family 9 cellulases from Clostridium cellulovorans (2006), Appl. Microbiol. Biotechnol., 71, 654-660.
    View publication on PubMed
Search for more literature for 3.2.1.4

Cloned(Commentary)

Cloned (Comment) Organism
-
 Clostridium cellulovorans

Protein Variants

Protein Variants Comment Organism
additional information EngHDELTACBM devoid of the carbohydrate binding module loses activity toward all substrates including carboxymethylcellulose Clostridium cellulovorans
additional information EngMDELTSACBM, devoid of the carbohydrate binding module has activity toward CMC (0.003 U/mg), in contrast with no activity toward any other substrate Clostridium cellulovorans
additional information EngYDELTSACBM, devoid of the carbohydrate binding module loses activity toward all substrates including carboxymethylcellulose Clostridium cellulovorans

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Clostridium cellulovorans O65987
-
-
Clostridium cellulovorans Q9AQF4
-
-
Clostridium cellulovorans Q9LAJ2
-
-
Clostridium cellulovorans Q9RGE6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Clostridium cellulovorans
EngH and truncated derivative Clostridium cellulovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acid-swollen cellulose + H2O
-
 Clostridium cellulovorans ?
-
 ?
acid-swollen cellulose + H2O cleaved cellulose randomly Clostridium cellulovorans ?
-
 ?
avicel + H2O
-
 Clostridium cellulovorans ?
-
 ?
beta-glucan + H2O
-
 Clostridium cellulovorans ?
-
 ?
carboxymethylcellulose + H2O
-
 Clostridium cellulovorans ?
-
 ?
cellohexaose + H2O
-
 Clostridium cellulovorans ?
-
 ?
cellooligosaccharides + H2O random cleavage, slow reaction rates for cellooligosaccharides smaller than G5 Clostridium cellulovorans ?
-
 ?
cellopentaose + H2O
-
 Clostridium cellulovorans ?
-
 ?
cellotetraose + H2O
-
 Clostridium cellulovorans ?
-
 ?
lichenan + H2O
-
 Clostridium cellulovorans ?
-
 ?

Synonyms

Synonyms Comment Organism
endoglucanase H
-
 Clostridium cellulovorans
endoglucanase L
-
 Clostridium cellulovorans
endoglucanase M
-
 Clostridium cellulovorans
endoglucanase Y
-
 Clostridium cellulovorans
EngH
-
 Clostridium cellulovorans
EngL
-
 Clostridium cellulovorans
EngM
-
 Clostridium cellulovorans
EngY
-
 Clostridium cellulovorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Clostridium cellulovorans
42
-
-
 Clostridium cellulovorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 stable below Clostridium cellulovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Clostridium cellulovorans
6.5
-
-
 Clostridium cellulovorans
7
-
-
 Clostridium cellulovorans
8
-
-
 Clostridium cellulovorans

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9 stable Clostridium cellulovorans
5 8 stable Clostridium cellulovorans