Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.4 extracted from

  • Hirasawa, K.; Uchimura, K.; Kashiwa, M.; Grant, W.D.; Ito, S.; Kobayashi, T.; Horikoshi, K.
    Salt-activated endoglucanase of a strain of alkaliphilic Bacillus agaradhaerens (2006), Antonie van Leeuwenhoek, 89, 211-219.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
CH3COONa 1.6 M, 4.45fold activation Salipaludibacillus agaradhaerens
NH4Cl 0.2 M, 3fold activation Salipaludibacillus agaradhaerens
NH4NO3 0.05 M, 2.8fold activation Salipaludibacillus agaradhaerens

General Stability

General Stability Organism
enzyme is stable to incubation with hydrogen peroxide Salipaludibacillus agaradhaerens

Inhibitors

Inhibitors Comment Organism Structure
Fe2+ 2 mM, 67% inhibition Salipaludibacillus agaradhaerens
Hg2+ 2 mM, 20% inhibition Salipaludibacillus agaradhaerens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 2 mM, 1.5fold activation Salipaludibacillus agaradhaerens
KCl 0.8 M, 4.23fold activation Salipaludibacillus agaradhaerens
LiCl 0.05 M, 1.9fold activation Salipaludibacillus agaradhaerens
Mg2+ 2 mM, 1.48fold activation Salipaludibacillus agaradhaerens
Na2SO4 0.5 M, 4.43fold activation Salipaludibacillus agaradhaerens
NaBr 0.5 M, 3.84fold activation Salipaludibacillus agaradhaerens
NaCl the enzyme activity increass around 4fold by the addition of 0.2-2.0 M NaCl in 0.1 M glycine-NaOH buffer (pH 9.0) Salipaludibacillus agaradhaerens
NaNO3 0.5 M, 4fold activation Salipaludibacillus agaradhaerens
Sr2+ 2 mM, 1.45fold activation Salipaludibacillus agaradhaerens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
x * 38000, SDS-PAGE Salipaludibacillus agaradhaerens

Organism

Organism UniProt Comment Textmining
Salipaludibacillus agaradhaerens
-
JAM-KU023
-
Salipaludibacillus agaradhaerens JAM-KU023
-
JAM-KU023
-

Purification (Commentary)

Purification (Comment) Organism
-
Salipaludibacillus agaradhaerens

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
Salipaludibacillus agaradhaerens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
avicel + H2O 33% of the activity with carboxymethyl cellulose Salipaludibacillus agaradhaerens ?
-
?
avicel + H2O 33% of the activity with carboxymethyl cellulose Salipaludibacillus agaradhaerens JAM-KU023 ?
-
?
carboxymethyl cellulose + H2O
-
Salipaludibacillus agaradhaerens ?
-
?
carboxymethyl cellulose + H2O
-
Salipaludibacillus agaradhaerens JAM-KU023 ?
-
?
lichenan + H2O 380% of the activity with carboxymethyl cellulose Salipaludibacillus agaradhaerens ?
-
?
lichenan + H2O 380% of the activity with carboxymethyl cellulose Salipaludibacillus agaradhaerens JAM-KU023 ?
-
?
additional information the enzyme does not degrade curdran, xylan, laminarin and pectin (0.5% each) at all Salipaludibacillus agaradhaerens ?
-
?
additional information the enzyme does not degrade curdran, xylan, laminarin and pectin (0.5% each) at all Salipaludibacillus agaradhaerens JAM-KU023 ?
-
?

Subunits

Subunits Comment Organism
? x * 38000, SDS-PAGE Salipaludibacillus agaradhaerens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
optimum without NaCl. Enzyme activity increases approximately 6fold at 60°C with 0.2 M NaCl compared to that at 50°C without NaCl in 0.1 M glycine-NaOH buffer (pH 9.0) Salipaludibacillus agaradhaerens
60
-
optimum with 0.2 M NaCl. Enzyme activity increases approximately 6fold at 60°C with 0.2 M NaCl compared to that at 50°C without NaCl in 0.1 M glycine-NaOH buffer (pH 9.0) Salipaludibacillus agaradhaerens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.4
-
carboxymethyl cellulose in presence of 25 mM Salipaludibacillus agaradhaerens
84.4
-
carboxymethyl cellulose in presence of 250 mM Salipaludibacillus agaradhaerens
190.1
-
carboxymethyl cellulose in presence of 25 mM Salipaludibacillus agaradhaerens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7 optimum without NaCl Salipaludibacillus agaradhaerens
7 9.4 optimum with 0.2 M NaC Salipaludibacillus agaradhaerens