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Literature summary for 3.2.1.4 extracted from
- Kinetic and structural optimization to catalysis at low temperatures in a psychrophilic cellulase from the Antarctic bacterium Pseudoalteromonas haloplanktis (2004), Biochem. J., 384, 247-253.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Dickeya chrysanthemi |
| expression in Escherichia coli | Pseudoalteromonas haloplanktis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.5 | - |
p-nitrophenyl-beta-D-cellobioside | 4°C | Dickeya chrysanthemi |  |
| 4 | - |
p-nitrophenyl-beta-D-cellobioside | 35°C | Dickeya chrysanthemi | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dickeya chrysanthemi | - |
- |
- |
| Pseudoalteromonas haloplanktis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Dickeya chrysanthemi |
- |
Pseudoalteromonas haloplanktis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture supernatant | - |
Dickeya chrysanthemi | - |
| culture supernatant | - |
Pseudoalteromonas haloplanktis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-nitrophenyl-beta-D-cellobioside + H2O | - |
Dickeya chrysanthemi | ? | - |
? | |
| p-nitrophenyl-beta-D-cellobioside + H2O | - |
Pseudoalteromonas haloplanktis | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cel5 | - |
Dickeya chrysanthemi |
| CelG | - |
Pseudoalteromonas haloplanktis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Pseudoalteromonas haloplanktis |
| 55 | - |
- |
Dickeya chrysanthemi |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
stable | Dickeya chrysanthemi |
| 45 | - |
half-life: 40 min | Pseudoalteromonas haloplanktis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
p-nitrophenyl-beta-D-cellobioside | - |
Dickeya chrysanthemi | |
| 0.18 | - |
p-nitrophenyl-beta-D-cellobioside | - |
Pseudoalteromonas haloplanktis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7 | - |
Dickeya chrysanthemi |
| 6.5 | 7 | - |
Pseudoalteromonas haloplanktis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | pH 4: about 35% of maximal activity, pH 8: about 45% of maximal activity | Dickeya chrysanthemi |
| 5 | 8 | pH 5: about 40% of maximal activity, pH 8: about 45% of maximal activity | Pseudoalteromonas haloplanktis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9.5 | optimally stable in this range | Pseudoalteromonas haloplanktis |
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